技术方法
近红外漫反射光谱法快速测定药用植物淫羊藿总黄酮含量
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- 1陕西师范大学食品工程与营养科学学院, 西北濒危药材资源开发国家工程实验室, 西安 710062
2西安交通大学医学院, 卫生部法医学重点实验室, 西安 710061
收稿日期: 2012-07-02
修回日期: 2012-09-06
网络出版日期: 2012-11-01
基金资助
中央高校基本科研业务费专项资金项目;大学生创新性实验计划项目
Rapid Determination of Total Flavonoids in a Medicinal Plant Epimedium by Near-infrared Reflectance Spectroscopy
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- 1National Engineering Laboratory of Endangered Medicinal Materials Resources Development in Northwest China, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China;
2Key Laboratory of Ministry of Health for Forensic Sciences, School of Medicine, Xi'an Jiaotong University, Xi’an 710061, China
Received date: 2012-07-02
Revised date: 2012-09-06
Online published: 2012-11-01
摘要
利用一种基于近红外漫反射光谱技术的淫羊藿总黄酮快速定量分析方法, 测定了淫羊藿属(Epimedium)7个种中总黄酮的含量。研究结果表明, 近红外光谱经二阶导数处理、主成分分析聚类及加权多元离散校正处理后, 采用改进最小二乘法回归得到的定标模型的预测效果最佳; 定标样品集预测方程的定标决定系数、交互验证标准差及交互验证相关系数分别为0.985 5、0.023 0和0.044 3; 检验样品集方程的定标决定系数、检验标准差及偏差分别为0.965 1、0.018和0.006, 达到了快速测定淫羊藿总黄酮含量的要求。该方法为淫羊藿总黄酮的快速定量分析提供了新的依据。
本文引用格式
薛耀碧, 张华峰, 杨晓华, 牛丽丽, 张翔, 刘冬, 李建科 . 近红外漫反射光谱法快速测定药用植物淫羊藿总黄酮含量[J]. 植物学报, 2013 , 48(1) : 65 -71 . DOI: 10.3724/SP.J.1259.2013.00065
Abstract
Epimedium is a famous medicinal plant in China. Total flavonoids in Epimedium exhibit a wide variety of bioactivities. We developed an analytical method based on near-infrared reflectance spectroscopy for determining total flavonoids in Epimedium and measured the content of total flavonoids in 7 Epimedium species. The best calibration model was under the following conditions: 2nd derivative, principal components analysis, weighted multiplicative scatter correction and modified partial least squares regression. The coefficient of determination for calibration, standard error of cross-validation and coefficient of determination for cross-validation of prediction equation of calibration set were 0.985 5, 0.023 0 and 0.044 3, respectively. The coefficient of determination, standard error of performance and bias of prediction equation of validation set were 0.965 1, 0.018 and 0.006, respectively. These results confirmed the good predictability of the model. The analytical method can be efficiently used for rapid quantification of total flavonoids in Epimedium.
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Zhang W, Chen H, Wang Z, Lan G, Zhang L (2012). Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai. Journal of Food Science and Technology (in press).
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孙翊, 李慧, 王亮生, 戴思兰 (2011). 一种快速有效分析烟草花冠中花青素苷的方法. 植物学报 46, 189-196.
张华峰, 高翔, 卢大炎, 王瑛 (2007). HPLC法同时测定淫羊藿中朝藿定A、B、C 与淫羊藿苷的含量. 分析测试学报 26, 198-201.
张华峰, 杨晓华 (2009). 淫羊藿药材质量控制的问题与对策. 中草药 40, 160-163.
张华峰, 杨晓华, 郭玉蓉, 王瑛 (2009). 药用植物淫羊藿资源可持续利用现状与展望. 植物学报 44, 363-370.
Chen X, Wu J, Zhou S, Yang Y, Ni X, Yang J, Zhu Z, Shi C (2009). Application of near-infrared reflectance spectroscopy to evaluate the lutein and b-carotene in Chinese kale. Journal of Food Composition and Analysis 22, 148-153.
Ma H，He X, Yang Y, Li M, Hao D, Jia Z (2011). The genus Epimedium: An ethnopharmacological and phytochemical review. Journal of Ethnopharmacology 134, 519-541.
Pei LK, Sun SQ, Guo BL, Huang WH, Xiao PG (2008). Fast quality control of Herba Epimedii by using Fourier transform infrared spectroscopy. Spectrochimica Acta Part A 70, 258-264.
Sheng MY, Chen QF, Yang QX (2008). Variation in icariin and flavonoid contents of barrenwort accessions native to Guizhou, China. Biochemical Systematics and Ecology 36, 719-723.
Woo YA, Kim HJ, Ze KR, Chung H (2005). Near-infrared (NIR) spectroscopy for the non-destructive and fast determination of geographical origin of Angelicae gigantis Radix. Journal of Pharmaceutical and Biomedical Analysis 36, 955-959.
Zhang B, Rong ZQ, Shi Y, Wu JG, Shi CH (2011b). Prediction of the amino acid composition in brown rice using different sample status by near-infrared reflectance spectroscopy. Food Chemistry 127, 275-281.
Zhang DW, Cheng Y, Wang NL, Zhang JC, Yang MS, Yao XS (2008b). Effects of total flavonoids and flavonol glycosides from Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts. Phytomedicine 15, 55-61.
Zhang HF, Yang TS, Li ZZ Wang Y (2008a). Simultaneous extraction of epimedin A, B, C and icariin from Herba Epimedii by ultrasonic technique. Ultrasonics Sonochemistry 15, 376-385.
Zhang HF, Yang XH (2012). Asian medicine: protect rare plants. Nature 482, 35-35.
Zhang W, Chen H, Wang Z, Lan G, Zhang L (2012). Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai. Journal of Food Science and Technology (in press).
Zhang YJ, Dang HS, Wang Y, Li XW, Li JQ (2011a). A taxonomic revision of unifoliolate Chinese Epimedium L. (Berberidaceae). Kew Bulletin 66, 253-262
Zhang YJ, Li JQ (2009). A New Species of Epimedium (Berberidaceae) from Hubei, China. Novon 19, 567-569.
Zhao HY, Sun JH, Fan MX, Fan L, Zhou L, Li Z, Han J, Wang BR, Guo DA (2008). Analysis of phenolic compounds in Epimedium plants using liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of Chromatography A 1190, 157-181.