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植物学报 >

2017 , Vol. 52 >Issue 5: 631 - 636

DOI: https://doi.org/10.11983/CBB16156

技术方法

茉莉酸及其前体12-氧-植物二烯酸的HPLC-MS/MS检测方法

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  • 1北京林业大学生物科学与技术学院, 国家林业局树木花卉育种与生物工程重点实验室, 北京 100083
    2南京农业大学农学院, 南京 210095

收稿日期: 2016-07-27

  录用日期: 2016-12-05

  网络出版日期: 2017-07-10

基金资助

北京市高等学校青年英才计划(No.YETP0755)

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Simultaneous Determination of Jasmonic Acid and 12-Oxo- Phytodienoic Acid in Different Plant Materials by HPLC-MS/MS

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  • 1The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of Chinese Forestry Administration, College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
    2College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2016-07-27

  Accepted date: 2016-12-05

  Online published: 2017-07-10

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摘要

茉莉酸是植物体内重要的伤反应特异激素, 含量极低, 但作用较大。当植物受到机械伤害时, 茉莉酸调控一系列应激反应。12-氧-植物二烯酸(OPDA)是茉莉酸合成途径中的重要前体, 与茉莉酸有密切的关系。该研究建立了一种HPLC-MS/MS分析检测方法, 可同时检测植物材料中茉莉酸及其前体OPDA含量。利用该方法建立的标准曲线具有良好的线性相关性, 相关系数达0.991 9-0.999 5, 回收率范围为91.91%-101.56%。该方法同时具有较好的检测灵敏度, 2种化合物的最低检测限为3.218和34.129 pmol。研究结果说明该方法切实可靠且具有较好的适用性。利用该方法检测了4种不同植物样品中目标化合物的含量, 结果表明该方法能够用于不同植物材料中这2种活性化合物含量的检测分析。

关键词： 茉莉酸; 12-氧-植物二烯酸; HPLC-MS/MS

本文引用格式

张双玉, 齐芪, 李晓慧, 盖颖, 蒋湘宁 . 茉莉酸及其前体12-氧-植物二烯酸的HPLC-MS/MS检测方法[J]. 植物学报, 2017 , 52(5) : 631 -636 . DOI: 10.11983/CBB16156

Abstract

Jasmonic acid is one of the important phytohormones responding to abiotic and biotic injury. Detecting the acid is difficult because of the low content in plant material. 12-oxo-phytodienoic acid is an important precursor in the biosynthesis of jasmonic acid. In this research, we established an HPLC-MS/MS method to determine the content of these two target chemicals simultaneously in 4 types of plant materials. An external standard curve was used to calculate the content in plant material. The regression coefficiency factor of the standard curve reached 0.991 9 to 0.999 5. Spiked recovery was from 91.91% to 101.56%. The method is sensitive and available; The limits of detection of two target chemicals were 3.218 and 34.129 pmol. This method was used to determinate the contents of these two target chemicals in 4 types of plant materials.

Key words： jasmonic acid; 12-oxo-phytodienoic acid; HPLC-MS/MS

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