应用SELEX技术检测水稻RFL转录因子的DNA结合位点
# 共同第一作者
收稿日期: 2016-06-13
录用日期: 2016-10-13
网络出版日期: 2017-01-23
基金资助
国家自然科学基金(No.31401324)
Use of SELEX for Research of the DNA Binding Sites of the RFL, a Transcription Factor of Rice
# Co-first authors
Received date: 2016-06-13
Accepted date: 2016-10-13
Online published: 2017-01-23
FLORICAULA/LEAFY是植物特有的转录因子, 其可以通过调控下游基因的表达对植物的发育起到调控作用。水稻(Oryza sativa)中的同源基因RFL (Rice FLORICAULA/LEAFY)能够调控多个发育过程, 包括开花、叶间期以及枝梗和颖花分化。指数富集配体的系统进化(SELEX)技术已经被广泛应用于体外筛选转录因子的DNA结合位点。应用SELEX技术检测水稻RFL的DNA结合序列, 经过7次PCR循环富集过程获得结合特异序列, 通过测序和MEME分析, 鉴定到RFL的DNA结合序列为(C/A)(C/T)NN(T/C/A)G(G/T)。实验结果为进一步阐明RFL分子功能奠定了基础。
丁承强, 常忠原, 王琰, 王绍华, 丁艳锋 . 应用SELEX技术检测水稻RFL转录因子的DNA结合位点[J]. 植物学报, 2017 , 52(1) : 70 -76 . DOI: 10.11983/CBB16130
FLORICAULA/LEAFY encodes an evolutionarily conserved land plant-specific transcription factor. It functions by regulating the expression of downstream genes. RFL, the FLORICAULA/LEAFY homolog in rice, controls several important developments in rice: flowering time, plastochron and panicle branching. In this study, we detected the binding specificity of RFL by using the method systematic evolution of ligands by exponential enrichment (SELEX). After seven SELEX processes and sequencing, the binding site of RFL was identified as (C/A)(C/T)NN(T/C/A)G(G/T). The result contributes to clarifying the molecular function of RFL.
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