以哥伦比亚生态型(Columbia-0)拟南芥(Arabidopsis thaliana)为实验材料, 人工模拟UV-B辐照处理拟南芥种子, 统计其发芽势和发芽率, 测定根长、株高、叶绿素、可溶性糖、可溶性蛋白以及丙二醛(MDA)含量, 研究UV-B辐照处理对拟南芥种子萌发和幼苗生长的影响。研究结果表明, 低剂量的UV-B辐照可以促进拟南芥种子萌发和幼苗生长, 并且最佳辐照剂量为1.0 kJ·m–2(P<0.05), 此时的发芽势、发芽率、根长、株高、叶绿素、可溶性糖及可溶性蛋白的含量均达到最大, 而丙二醛的含量变化则不明显(P>0.05)。当辐照剂量大于1.0 kJ·m–2时, 促进作用逐渐变小, 并且随着辐照剂量的增加, 表现出了抑制作用。实验结果表明, 适当剂量的UV-B辐照在一定程度上可以促进拟南芥种子萌发和幼苗生长, 而过高剂量的辐照则对其产生抑制或损伤作用。
To explore the impact of UV-B irradiation on Arabidopsis seed germination and seedling growth, we treated seeds of Columbia wild-type Arabidopsis with artificial UV-B and examined germination potential, germination percentage, root length, plant height, and contents of chlorophyll, soluble sugar, soluble proteins and malonaldehyde (MDA). A short period of UV-B irradiation promoted Arabidopsis growth, with a significant effect at 1.0 kJ·m–2 (P<0.05), with peak germination potential and percentage, as well as root length, plant height, and contents of chlorophyll, soluble sugar and soluble proteins. However, MDA content was not affected (P>0.05). With doses > 1.0 kJ·m–2, the promotion was reduced and was inhibited with longer irradiation. Thus, suitable UV-B irradiation dose could promote seed germination and seedling growth of Arabidopsis, and a too-high dose is an inhibitor, inducing damage.
韩榕,王勋陵,岳明等（2002）.增强UV-B辐射对小麦体细胞分裂的影响.遗传学报 29(6):
537-541.
Gilbert M, Pors Y, Grover K, Weingart I, Skotnica J, Grimm B, Seidlitz H K, Langebartels C, Wilhelm C(2009). Intra-and interspecific differences of 10 barley and 10 tomato cultivars in response to short-time UV-B radiation: A study analysing thermoluminescence, fluorescence, gas-exchange and biochemical parameters. Environ Pollut,157: 1603-1612.
Wang S W, Xie B T, Yin L N, Duan L S, Li Z H, Eneji A E, Tsuji W, Tsunekawa A（2010）. Increased UV-B radiation affects the viability, reactive oxygen species accumulation and antioxidant enzyme activities in maize (Zea mays L.) pollen.Photochem Photobiol, 86(1)：110-116
郭爱华,高丽美,李永锋等（2010）.增强紫外线B辐射对小麦根尖细胞微管骨架的影响.中国细胞生物学学报. 32(2):256-260.
翟菁如,陈慧泽,高丽美等（2010）.增强UV-B辐射对小麦叶肉细胞原生质体微丝骨架的影响.生物学杂志. 27(6),33-36.
陈璋(1994).拟南芥：植物分子生物学研究的模式物种.植物学通报.11（1）：6-11.
张志良,瞿伟青等（1990）.植物生理学实验指导.北京:高等教育出版社.pp.88-91．
李合生等（2000）.植物生理生化实验原理和技术.北京:高等教育出版社.pp.260,185,134.
冯虎元,徐世健,安黎哲等（2001）.UV-B辐射对8个大豆品种种子萌发率和幼苗生长的影响. 西北植物学报.21(1):14-20.
冯虎元, 安黎哲,陈书燕等（2002）.增强UV-B辐射与干旱复合处理对小麦幼苗生理特性的影响.生态学报, 22(9):1564-1568.
Sullivan J H, Teramura A H, Dillenburg L R(1994). Growth and photosynthetic responses of field-grown sweetgum (Liquidambar styraciflua, Hamamelidaceae) seedlings to UV-B radiation. Am J Bot, 81(7):826-832.
侯扶江,贲桂英,韩发等（1998）.田间增加紫外线（UV）辐射对大豆幼苗生长和光合作用的影响.植物生物学报, 22(3):256-261.
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