拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应

王翠丽, 田海霞, 汪姣姣, 齐天从, 黄煌, 任春梅, 谢道昕, 宋素胜

doi:10.3724/SP.J.1259.2014.00643

植物学报 >

2014 , Vol. 49 >Issue 6: 643 - 652

DOI: https://doi.org/10.3724/SP.J.1259.2014.00643

研究报告

拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应

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¹湖南农业大学生物科学技术学院, 长沙 410128;
²清华大学生命科学学院, 清华北大生命科学联合中心, 北京 100084

收稿日期: 2013-09-24

修回日期: 2014-06-02

网络出版日期: 2014-11-21

基金资助

国家自然科学基金;国家自然科学基金;中国博士后科学基金特别资助;中国博士后科学基金特别资助

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bHLH17 Negatively Regulates Jasmonate-mediated Plant Defense Responses

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¹College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

²Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China

Received date: 2013-09-24

Revised date: 2014-06-02

Online published: 2014-11-21

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摘要

植物激素茉莉素作为抗性信号调控植物对腐生性病原菌和昆虫的抗性, 作为发育信号调控植物根的生长、雄蕊发育、表皮毛形成和叶片衰老。茉莉素受体COI1识别茉莉素分子, 进而与JAZ蛋白互作并诱导其降解, 继而调控多种茉莉素反应。拟南芥(Arabidopsis thaliana) IIId亚组bHLH转录因子(bHLH3、bHLH13、bHLH14和bHLH17)是JAZ的一类靶蛋白。与野生型相比, IIId亚组bHLH转录因子的单突变体对灰霉菌和甜菜夜蛾的抗性无明显差异, 而四突变体对灰霉菌和甜菜夜蛾的抗性增强。该文通过高表达bHLH17并研究其对灰霉菌和甜菜夜蛾的抗性反应, 结果显示, 被灰霉菌侵染的bHLH17高表达植株较野生型表现出更严重的病症。取食bHLH17高表达植株叶片的甜菜夜蛾幼虫体重大于取食野生型叶片的幼虫体重。bHLH17高表达抑制了茉莉素诱导的抗性相关基因(Thi2.1)和伤害响应基因(VSP2、AOS、JAZ1、JAZ9和JAZ10)的表达。原生质体转化实验显示bHLH17通过其N端行使转录抑制功能。研究结果表明, IIId亚组bHLH转录抑制因子bHLH17高表达会负调控茉莉素介导的对灰霉菌和甜菜夜蛾的抗性。

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王翠丽, 田海霞, 汪姣姣, 齐天从, 黄煌, 任春梅, 谢道昕, 宋素胜 . 拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应[J]. 植物学报, 2014 , 49(6) : 643 -652 . DOI: 10.3724/SP.J.1259.2014.00643

Abstract

The plant hormone jasmonate (JA) functions as a defense signal to regulate plant defense against insect attack and pathogen infection. It also acts as a developmental signal to regulate plant development and growth, including root growth, stamen development, trichome initiation, and leaf senescence. The JA receptor CORONATINE INSENSITIVE1 (COI1) perceives JA signals for interaction with JASMONATE-ZIM-DOMAIN (JAZ) proteins and subsequent ubiquitination of JAZs, thereby regulating the JA responses. The single mutants of the bHLH subgroup IIId factors (bHLH3, bHLH13, bHLH14 and bHLH17), which interact with JAZs, show no obvious phenotype in resistance to the fungus Botrytis cinerea and defense against the herbivore Spodoptera exigua. However, the quadruple mutant shows enhanced resistance to B. cinerea and defense against S. exigua. We investigated whether the overexpression of bHLH17 in Arabidopsis affected the resistance to B. cinerea and defense against S. exigua. Similar to the JA receptor mutant coi1-1, Arabidopsis bHLH17-overexpressing plants were more susceptible to B. cinerea and S. exigua than the wild type. As compared with the wild type, bHLH17 overexpression attenuated the JA-inducible expression of defense- or wounding-related genes, such as Thi2.1, VSP2, AOS, JAZ1, JAZ9 and JAZ10. Further transient expression analysis revealed that the N-terminus of bHLH17 was essential for its transcriptional repression function. Overexpression of bHLH17 negatively regulates plant defense against B. cinerea and S. exigua.

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