利用兼并性引物和RACE方法, 在南蛇藤(Celastrus orbiculatus)中克隆了1个脯氨酸脱氢酶基因, 并命名为NstProDH1。序列比对显示该基因与拟南芥(Arabidopsis thaliana)和烟草(Nicotiana tabacum)的脯氨酸脱氢酶(ProDH)具有很高的同源性。酶学特性分析表明该酶具有脯氨酸脱氢酶的活性。比较南蛇藤不同器官该基因的转录表达模式与脯氨酸脱氢酶活性, 结果显示两者之间没有明显的关联, 说明该基因的表达受到转录和翻译水平的双重调控, 同时也暗示南蛇藤中还存在其它的脯氨酸脱氢酶基因。NstProDH1基因的表达模式与野生型拟南芥中的ProDH1具有相似性, 因此推测NstProDH1基因可能在功能上与拟南芥ProDH1基因相似。
We cloned a proline dehydrogenase gene from Celastrus orbiculatus using degenerate primers and RACE and named it NstProDH1. Sequence alignment showed that NstProDH1 has high sequence similarity with corresponding genes from Arabidopsis and tobacco. Enzymatic characteristic analysis identified the activity of proline dehydrogenase. Comparison of gene expression patterns and proline dehydrogenase activity were not associated in different organs, so NstProDH1 was regulated by transcription and translation. The gene expression pattern of NstProDH1 was similar to that of Arabidopsis ProDH1, therefore NstProDH1 may be functionally similar to Arabidopsis ProDH1.
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