为研究外源硫化氢(H2S)对豌豆(Pisum sativum)胚根生理特性及其边缘细胞的影响, 测定了不同浓度外源H2S处理下豌豆根长、根尖组织可溶性蛋白质含量、抗氧化酶(APX、CAT、POD和SOD)活性、根尖边缘细胞存活率及其粘胶层相对面积的变化。结果表明: 低浓度(0–40 μmol·L–1)H2S可促进豌豆胚根生长, 根尖组织可溶性蛋白质含量升高, SOD、APX和POD活性增加, CAT活性降低, 根尖边缘细胞存活率上升, 粘胶层相对面积变小。高浓度(60–80 μmol·L–1)H2S可抑制豌豆胚根生长, 可溶性蛋白质含量和边缘细胞存活率明显下降, APX和POD活性降低, CAT活性升高, SOD活性没有明显变化, 粘胶层相对面积变大; 边缘细胞染色体凝集并边缘化, 然后逐渐降解并伴随粉末化, 细胞质膜皱缩。因此, 推测H2S可能在植物体内发挥着重要的生理作用。
To further study the effect of exogenous hydrogen sulfide (H2S) on the physiological character of the radicle and its border cells of Pisum sativum, we investigated the variation in root tip and root border cells and other indexes after H2S treatment at different concentrations. We measured root lengths, content of soluble protein in root tip tissues, antioxidant enzyme activities (APX, CAT, POD and SOD), survival rate of root border cells, and relative area of the mucilage layer. A low concentration of H2S (0–40 μmol·L–1) could increase the embryo root length of P. sativum; content of root tip soluble protein; activity of SOD, APX and POD; and survival rate of root border cells. This concentration decreased the activity of CAT and the relative area of the mucilage layer. When the concentration of H2S was increased from 60 to 80 μmol·L–1, embryo root length was reduced; content of soluble protein of root tips and survival rate of border cells were rapidly reduced; and activity of APX and POD was gradually reduced; but CAT activity was significantly increased, with no effect on SOD activity. Moreover, with improvement in relative area of the mucilage layer, the chromosomes of the root border cells were gradually condensed, marginalized and degraded; furthermore, the plasma membrane was condensed. Therefore, H2S might have an important role in the physiological process of plants.
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