技术方法

应用稀土调控藏红花胚性愈伤组织的生长与分化

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  • 1中国科学院过程工程研究所生化工程国家重点实验室, 北京 100190;
    2科技部生物中心, 北京 100036

收稿日期: 2009-10-23

  修回日期: 2009-12-23

  网络出版日期: 2010-09-20

Regulating the Cell Growth and Shoot Induction of Crocus sativus Embryogenic Callus by Rare Earth Elements

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  • 1The State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;

    2The China National Center for Biotechnology Development, Ministry of Science and Technology, Beijing 100036, China

Received date: 2009-10-23

  Revised date: 2009-12-23

  Online published: 2010-09-20

摘要

为提高藏红花(Crocus sativus)胚性愈伤组织的繁殖系数与出芽率, 以建立藏红花离体快繁体系, 解决其资源短缺问题, 采用两步法, 用稀土调控其胚性愈伤组织的生长与分化。结果表明, 在添加了0.25 mg·L–1 NAA、3 mg·L–1 6-BA和400 mg·L–1 CH的B5固体培养基中, 0.04 mmol·L–1 La3+促进胚性愈伤组织生长的效果最佳, 繁殖系数为12, 是不添加稀土处理组的1.48倍; 在添加了0.25 mg·L–1 NAA、3 mg·L–1 6-BA和400 mg·L–1 CH的1/2 B5固体培养基中, 0.06 mmol·L–1 Ce3+促进胚性愈伤组织分化出芽的效果最佳, 出芽率高达84.5%, 是不添加稀土处理组的1.81倍, 且高于国外报道的出芽率(40%)。初步解决了藏红花胚性愈伤组织生长慢和出芽率低等问题, 为建立高效稳定的藏红花离体快繁体系奠定了基础。

本文引用格式

陈书安, 王晓东, 赵兵, 王玉春 . 应用稀土调控藏红花胚性愈伤组织的生长与分化[J]. 植物学报, 2010 , 45(05) : 609 -614 . DOI: 10.3969/j.issn.1674-3466.2010.05.011

Abstract

Rare earth elements were used to promote the growth and induction of embryogenic callus of Crocus sativus by two-stage method for its micropropagation in vitro and then to solve the problems of its limited availability. For promoting cell growth, embryogenic calli were cultured on B5 solid medium supplemented with 0.25 mg·L–1 NAA, 3 mg·L–1 6-BA, 400 mg·L–1 CH, and 0.04 mmol·L–1 La3+. The maximal proliferation rate was 12, which was 1.48-fold of that without rare earth elements. For promoting shoot induction, embryogenic calli were cultured on 1/2 B5 solid medium supplemented with 0.25 mg·L–1 NAA, 3 mg·L–1 6-BA, 400 mg·L–1 CH, and 0.06 mmol·L–1 Ce3+. The maximal shoot induction rate was 84.5%, which was 1.81-fold of that without rare earth elements. In this study, rare earth elements could enhance the cell growth and shoot induction rate of embryogenic calli of C. sativus, which would be useful for its micropropagation.
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