分析了146份种质资源的形态学性状, 同时利用SSR荧光分子标记分析了其遗传多样性和群体遗传结构。形态学性状多样性分析结果表明, 质量性状和数量性状的变异系数变化范围分别为8.22%~267.58%与14.35%~72.51%, 多样性指数变化范围分别为为0.04~1.91与1.58~2.02, 说明辣椒种质资源多样性丰富。利用SSR荧光分子标记22对引物共检测出102个等位基因, 平均每对引物检测出4.636个等位基因, 有效等位变异基因变化范围为1.191~5.311, 香农指数数值范围为0.345~2.056, 多态性信息含量(PIC)数值范围为0.153~0.795, 146份辣椒种质资源平均遗传距离为0.429。基于表型和分子标记的聚类分别将辣椒种质资源分为6类和7类, 但两种聚类结果相关性较弱(r=0.3967)。群体遗传结构分析将辣椒种质从群体遗传结构分为2组, 不同组界限明显。研究阐明了146份辣椒种质资源的遗传多样性和群体遗传结构, 为后续进行种质鉴定、新品种选育奠定了理论基础。
杨志刚
,
张鹏程
,
常海文
,
康立茹
,
左毅
,
向浩鑫
,
韩凤英
. 基于形态学性状和SSR荧光分子标记的辣椒种质资源遗传多样性分析[J]. 植物学报, 2025
, 60(2)
: 1
-0
.
DOI: 10.11983/CBB24098
The genetic diversity and population structure of 146 pepper germplasm resources were analyzed based on morphological traits and SSR fluorescent molecular markers. The results of phenotypic trait diversity analysis showed that the coefficient of variation of quality traits and quantitative traits ranged from 8.22% to 267.58% and 14.35% to 72.51%, respectively, and the genetic diversity index ranged from 0.04 to 1.91 and 1.58 to 2.02, respectively. The genetic diversity of pepper germplasm resources was rich. A total of 102 alleles were detected by 22 pairs of SSR fluorescent molecular markers, with an average of 4.636 alleles per pair of primers. The effective allelic variation gene ranged from 1.191 to 5.311, the Shannon index ranged from 0.345 to 2.056, and the polymorphic information content (PIC) ranged from 0.153 to 0.795.The average genetic distance of 146 pepper germplasm resources was 0.429. Based on phenotypic and molecular marker clustering, pepper germplasm resources were divided into six and seven groups, respectively, but the correlation between the two clustering results was weak (r=0.3967). The population structure analysis divided the pepper germplasm resources into two groups, and the boundaries of different groups were obvious. In this study, the genetic diversity and population structure of 146 pepper germplasm resources were described in detail, which provided a theoretical basis for germplasm identification and new variety breeding.
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