[an error occurred while processing this directive]

Plant Diversity ›› 2002, Vol. 24 ›› Issue (04): 1-3.

• 研究论文 •    

七种不同抗冷性植物甘油-3-磷酸转酰酶mRNA二级结构研究

柳维波,曹槐,刘世熙,刘次全   

  1. 云南大学现代生物学研究中心
  • 出版日期:2002-08-25 发布日期:2002-08-25

Study on the Predicted mRNA Secondary Structures of Plant Glycerol-3-Phosphate Acyltransferases

LIU Wei-Bo,CAO Huai,LIU Shi-Xi,LIU Ci-Quan   

  1. Research Center for Modern Biology,Yunnan University
  • Online:2002-08-25 Published:2002-08-25

摘要: 对南瓜、豌豆、黄瓜、拟南芥菜、红花、菠菜、黑子南瓜等7种不同抗冷性植物甘油-3-磷酸酰基转移酶的mRNA序列作了三核苷酸碱基模式分析,并用Zuker方法对其mRNA序列的翻译区进行了二级结构分析,统计出发夹、内环、膨胀环、三分支环、四分支环等二级结构的基本结构单元数,通过对编码脯氨酸的密码子在mRNA二级结构中的分布位置的研究,发现这些密码子主要是分布在茎的末端、环的根部、膨胀环或分支环上,占到了序列中所有编码脯氨酸的密码子总数的69% 到97% 的比例,这与我们在研究其他物种的mRNA二级结构时的发现是一致的。

关键词: GPAT酶, 抗冷性, mRNA二级结构, 脯氨酸

Abstract: 3-nucletide sequence modes were extracted from the mRNA sequences of glycerol-3-phosphate acyltransferases(GPAT)from different chill-resistance plants,squash(Cucurbita moschata),pea(Pisum sativum),cucumber(Cucumis sativus),Arabidopsis thaliana,redflow(Carthamus tinctorius),spinach(Spinacia oleracea)and figleaf gourd(Cucurbita ficifolia).The mRNA secondary structures of translation domains in the reported sequences were predicted using Zuker's prediction,respectively.

Key words: GPAT