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Plant Diversity ›› 2002, Vol. 24 ›› Issue (04): 1-3.

• 研究论文 •    

应用ITS区序列对秋海棠属无翅组分类学问题的探讨

向建英 管开云 杨俊波   

  1. 中国科学院昆明植物研究所
  • 出版日期:2002-08-25 发布日期:2002-08-25

Studies on the Classiication of Begonia Sect.Sphenanthera Based on Nuclear Ribosomal DNA ITS Sequence Data

XIANG Jian-Ying,GUAN Kai-Yun,YANG Jun-Bo   

  1. Kunming Institute of Botany,Chinese Academy of Sciences
  • Online:2002-08-25 Published:2002-08-25

摘要: 以侧膜组(Sect.Coeloecentrum)的Begonia cirrosa和单裂组(Sect.Reichenheimia)的Bhenryi为外类群,对秋海棠属(Begonia L.)无翅组(Sect.Sphenanthera)9种2变种及秋海棠组(Sect.Begonia)4种进行了ITS区(包括5.8S rDNA) 序列的测定。发现该属的ITS序列较一般类群要长一些,所测的序列长度变化范围为648~743bp。这些序列经Clustal X软件排序后序列总长为756个位点, gap做缺失处理时,变异位点和信息位点分别占26.41%和10.11%。运用PAUP4.0b8软件分析,获得6棵最大简约树,步长为286,一致性指数(CI)和维持性指数(RI)分别为0.8287和0.7841,利用6棵最简约树获取严格一致树,并作Bootstrap检测。结果表明:1. 支持将原无翅组内子房3室的种类移入秋海棠组,而将子房4室的种保留,成立四室组(Sect.Sphenanthera);2. 将Btetragona作为Bacetosella的异名处理得到一定的支持;3. Bcrassirostris的系统位置有待进一步的研究。

关键词: 秋海棠属, 无翅组, 四室组, ITS区

Abstract: The sequences of nrDNA internal transcribed spacers(ITS)(including 5.8S rDNA)of 9 species and 2 varieties in Sect.Sphenanthera,4 species in Sect.Begonia were examined and analyzed B.cirrosa (sect.Coelocentrum),B.henryi(Sect.Reichenheimia)as outgrorp.The length of these sequences is 648-743bp,and the aligned length of ITS region is 756 position,which provides 26.41% variable sites and 10.11%phylogenetical informative sites when gaps are treated as missing.Six most parsimonious trees,with length of 286,consistency index(CI)of 0.8287 and retention in dex(RI)of 0.7841 were obtained by PAUP 4.0b8 analysis.

Key words: Begonia L.:Sect.Sphenanthera:ITS region