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Plant Diversity ›› 2002, Vol. 24 ›› Issue (04): 1-3.

• 研究论文 •    

黄瓜和绿豆下胚轴不定根发生的研究

王利琳,庞基良,胡江琴,梁海曼   

  1. 浙江大学生命科学学院
  • 出版日期:2002-08-25 发布日期:2002-08-25

Studies on Adventitious Root Formation in Hypocotyls of Cucumber and Phaseolus Cultures in Vitro

WANG Li-Lin,PANG Ji-Liang,HU Jiang-Qin,LIANG Hai-Man   

  1. College of Life Sciences,Zhejiang University
  • Online:2002-08-25 Published:2002-08-25

摘要: 在MS基本培养基上,黄瓜和绿豆幼苗的下胚轴切段培养4d时即可见不定根发生。下胚轴不同部位切段的发根能力不同。下胚轴切段反插时比正插时发根快1~2d,发根率也高于正插的;0.01~0.05mg/L的NAA还诱导下胚轴切段在形态学上端发根。TIBA对正插或反插的下胚轴切段的不定根发生都有抑制作用。结果提示,生长素极性运输活性对不定根形成起着重要作用。

关键词: 不定根, 下胚轴, IAA极性运输, 黄瓜, 绿豆

Abstract: Hypocotyls from Cucumber and Phaseolus inoculated on Murashige and Skoog(MS)culture medium for 4 days,formed adventitious roots on morphologically lower side.The rooting capacity of each segment derived from three regions of each hypocotyl was different.The rooting speed on the morphologically lower side of segments which were inserted upside down is faster for one day to two days than which were inserted upright in the culture medium.

Key words: Adventitious root formation