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Plant Diversity ›› 1994, Vol. 16 ›› Issue (03): 1-3.

• 研究论文 •    

金花茶花药愈伤组织的体细胞减数分裂

李懋学 尤瑞麟 白文力 程金水   

  1. 北京大学生物系
  • 出版日期:1994-06-25 发布日期:1994-06-25

SOMATIC MEIOSIS OF ANTHER CALLUS CULTURE IN CAMELLIA PETELOTII

LI Mao-Xue YOU Rui-Lin BAI Wen-Li CHEN Jmg-Shui   

  1. Department of Biology, Peking University
  • Online:1994-06-25 Published:1994-06-25

摘要: 金花茶(Camellia petelotii)的小孢子单核期花药,经培养和继代培养6个月后的愈伤组织中,发现有少量体细胞进行减数分裂。在减数第一和第二次分裂中,同源染色体的配对和分离基本正常,最后形成四分体。该愈伤组织经石蜡切片和压片观察,发现其主要由大量的液化细胞和贮藏细胞所组成,此外,还有少部分分生细胞。没有发现进一步的分化。其染色体数目,2n=30者占71.7%其余则为非整倍体。

关键词: 金花茶, 花药愈伤组织, 体细胞减数分裂

Abstract: It was found that a few cells progress meiosis in an anther callus culture of Camellia petelotii (Merr.)Sealy cultured and subcultured for 6 months. The pairing and segregating of homologous chromosomes at the frist and second division of meiosis were conformed with PMC meiosis, and finally, the tetrad were formation. Basec on the analysis of chromosomal morphology and behavior, it was considered as a special somatic meiosis. The speciality and significance of this somatic meiosis were analyzed and discussed. Observation on preparations made with paraffin sectioning and squashing showed that callus clumps composed of some meristematic cell groups, a large quantity of vaculated cells and storge cells, without further differentiation. About 71.1 percent of cells in this callus clumps showed the chromosome number 2n = 30, and the rest cells were aneuploid.

Key words: Camellia petelotii