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Plant Diversity ›› 1994, Vol. 16 ›› Issue (03): 1-3.

• 研究论文 •    

中国野生葡萄葛*叶绿体DNA质粒文库的构建

吴俊辉 舒煦 查晓 李朝銮 余平   

  1. 四川省肿瘤研究所
  • 出版日期:1994-06-25 发布日期:1994-06-25

CONSTRUCTURE OF THE PLASMID LIBRARY OF cpDNA FROM VITIS FLEXUOSA IN CHINA

WU Jun-Hui SHU Xu ZHA Xiao LI Chao-Luan YU Ping   

  1. Sichuan Institute of Tumour
  • Online:1994-06-25 Published:1994-06-25

摘要: 限制性内切酶BamHI水解中国野生葡萄葛*(Vitis flexuosa Thunb)的叶绿体DNA(cpDNA),电泳分离为34条带,最大为11.7kb,最小为0.23kb,分别用pUC和pBR322质粒克隆所得片段,转化大肠杆菌,并从转化菌中提取质粒DNA,经酶切电泳分析证明含有葛田cpDNA经BamHI水解的不同片段,从而构建了葛*cpDNA的BamHI质粒文库。

关键词: 质粒文库.葛*、叶绿体DNA

Abstract: Choroplast DNA from Vitis fiexuosa Thunb was digested with BamHI. The resulting fragments were cloned into plasmids pUC7, pUC9 and pBR.322. The plasmid library of the BamHI fragments ofcpDNA from Vitis fiexuosa Thunb was obtained.

Key words: Plasmid library