植物学报 ›› 2009, Vol. 44 ›› Issue (05): 587-593.DOI: 10.3969/j.issn.1674-3466.2009.05.009

• 研究报告 • 上一篇    下一篇

光合菌对黄瓜光合及抗氧化同工酶的影响

宋兴舜1, 2, 任静3, 刘雪梅1, 2, 马双1, 杨传平2

  

  1. 1 东北林业大学生命科学学院遗传学科, 哈尔滨 150040; 2 林木遗传改良与生物技术教育部重点实验室(东北林业大学), 哈尔滨 150040; 3 东北农业大学成栋学院生物食品系, 哈尔滨 150030
  • 收稿日期:2008-12-18 修回日期:2009-02-22 出版日期:2009-09-01 发布日期:2009-09-01
  • 通讯作者: 杨传平

Effects of Photosynthetic Bacteria on Photosynthesis and Antioxidative Isoenzymes in Cucumber (Cucumis sativus)

Xingshun Song1, 2,Jing Ren3, Xuemei Liu1, 2, Shuang Ma1,Chuanping Yang2

  

  1. 1Department of Genetics, College of Life Science, Northeast Forestry University, Harbin 150040, China; 2Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, Harbin 150040, China
  • Received:2008-12-18 Revised:2009-02-22 Online:2009-09-01 Published:2009-09-01
  • Contact: Chuanping Yang

摘要:

以2种基因型的黄瓜(Cucumis sativus)为材料, 研究光合菌(PSB)的喷施对植物生物量、净光合速率(Pn)、叶片PSII 的最大光化学效率(Fv/Fm)及抗氧化同工酶代谢的影响。结果表明, 喷施PSB均能诱导2种基因型黄瓜的生物量显著增加, 并伴随Pn的显著提高。但是, 2种基因型黄瓜的Fv/Fm并不受PSB喷施的影响; PSB能使总过氧化物歧化酶(SOD)和抗坏血酸过氧化酶(APX)活性提高, 并使它们的多数同工酶的活性上调, 这些同工酶活性的增加在叶绿体中表现更为明显(如 Cu/Zn-SOD、Fe-SOD 和 sAPX)。研究结果表明, PSB能通过增强黄瓜抗氧化酶体系的活性改善植株的抗氧化能力, 从而在植株的生长和光合作用方面起到促进作用。

Abstract:

We examined the net photosynthetic rate (Pn), the maximal photochemical efficiency of PSII (Fv/Fm) and the activities of antioxidant isoenzymes in cucumber (Cucumis sativus) w ith or without photosynthetic bacteria (PSB) application. PSB spraying resulted in a significant increase in fresh weight and leaf area for both genotypes of cucumber plants, accompanied by a marked increase of Pn. However, Fv/Fm showed negligible changes. Exogenous PSB also increased activities of total superoxide dismutase (SOD) and ascorbate peroxidase (APX) , and a general increase of their isoenzymes. The increased level in Cu/Zn-SOD, Fe-SOD
and stroma APX (sAPX) was most significant in chloroplasts. These results implied that PSB played a favorable role in growth and photosynthesis and improved hardiness in cucumber by enhancing antioxidant enzyme activities.