%A Sun Yingkun, Hu Shaoqing, Pang Jiliang, Gao Kai, Liu Huahong, Chen Huanwei, Yao Tao, Chen Linjing, Shen Bochun %T Establishment of a Tissue Culture and Propagation System for Ardisia violacea, a Rare and Endangered Species %0 Journal Article %D 2017 %J Chinese Bulletin of Botany %R 10.11983/CBB16233 %P 764-773 %V 52 %N 6 %U {https://www.chinbullbotany.com/CN/abstract/article_3132.shtml} %8 2017-11-01 %X

Shoot segments with axillary buds from young branches of wild Ardisia violacea with excellent characters were cultured in vitro. Through bud induction, multiple shoots proliferation, subculture, rooting and transplantation, a regeneration system was established. The optimal bud induction medium was MS+0.80 mg?L-1 KT+0.10 mg?L-1 NAA+0.10 mg?L-1 IBA, and the germination frequency of axillary buds reached 92.60%; the optimal multiple shoot proliferation medium was MS1+0.50 mg?L-1 TDZ+0.10 mg?L-1 NAA, and the average proliferation coefficient reached 8.60; the optimal subculture medium was MS+1.00 mg?L-1 KT+0.50 mg?L-1 NAA; the optimal rooting medium was 1/2MS+2.00 mg?L-1 IBA+1.00 mg?L-1 NAA+1.00 mg?L-1 AC, and the average frequency of shoot rooting reached 98.70%. Using pine bark:peat (2:1, v/v) as a matrix, the transplant survival rate could reach 85.30%. We established a high-frequency regeneration system for A. violacea that could meet the needs of large-scale production.