%A Yujing Wang, Minchun Li, Wang Wu, Hanying Wu, Yinong Xu
%T Cloning and Characterization of an AP2/EREBP Gene TmAP2-1 from Tetraena mongolica
%0 Journal Article
%D 2013
%J Chinese Bulletin of Botany
%R 10.3724/SP.J.1259.2013.00023
%P 23-33
%V 48
%N 1
%U {https://www.chinbullbotany.com/CN/abstract/article_3083.shtml}
%8 2013-01-01
%X AP2/EREBP transcription factors play important roles in plant morphology, development, and stress responses. We cloned and characterized a putative AP2/ERF domain-containing gene from Tetraena mongolica by homology-based cloning and rapid amplification of cDNA ends. This gene, designated TmAP2-1 (accession no. JQ676996), encodes a protein of 483 amino acids with 2 copies of AP2 DNA binding domain. Subcellular localization analysis revealed that TmAP2-1 protein localized in the nucleus. TmAP2-1 protein has no transactivation activity in yeast. The expression of TmAP2-1 is induced by cold, salt, polyethylene glycol and abscisic acid. TmAP2-1 is detected in roots, shoots and leaves of T. mongolica, with the highest expression in leaves. Overexpression of TmAP2-1, accumulation of more soluble sugars in T. mongolica callus, but in reduced lipid content. TmAP2-1 probably represents an AP2/EREBP gene that plays a regulatory role in tolerance of T. mongolica to abiotic stresses by affecting accumulation of soluble sugars.