%A Shixuan Chen, Zhennan Zhang, Bo Wang, Yan Zhu, Yuehua Gong, Dongmei Sun, Xin Deng %T Cloning, Expression and Functional Analysis of a J-domain Protein- coding Gene, BhDNAJC2, from the Resurrection Plant Boea hygrometrica %0 Journal Article %D 2015 %J Chinese Bulletin of Botany %R 10.3724/SP.J.1259.2015.00180 %P 180-190 %V 50 %N 2 %U {https://www.chinbullbotany.com/CN/abstract/article_2967.shtml} %8 2015-03-01 %X Heat shock proteins (Hsps) can relieve environmental stress damage by aiding proper folding or degradation of denatured proteins. The resurrection plant Boea hygrometrica can tolerate desiccation and revive after rehydration. In desiccated leaves of B. hygrometrica, an Hsp-coding gene was cloned and designated BhDNAJC2. Sequence analysis revealed that BhDNAJC2 contained a J-domain and thus was a novel member of the Hsp40 family. Real-time PCR revealed that BhDNAJC2 was induced by dehydration, cold, heat, oxidative, alkaline stresses and abscisic acid treatment. Confocal microscopy revealed the BhDNAJC2-YFP fusion protein in the endoplasmic reticulum, nucleus and cytoplasm. Compared to the wild type, Arabidopsis plants overexpressing BhDNAJC2 showed improved stress tolerance under drought, heat, salt and alkaline stresses, as indicated by better growth, higher Fv/Fm, and lower electrolytic leakage. BhDNAJC2 likely plays a key role in resistance to osmotic, saline, alkaline and heat stresses in B. hygrometrica.