%A Wang Ying, Li Xianggan, Qiu Lijuan %T Research Progress in Off-target in CRISPR/Cas9 Genome Editing %0 Journal Article %D 2018 %J Chinese Bulletin of Botany %R 10.11983/CBB18004 %P 528-541 %V 53 %N 4 %U {https://www.chinbullbotany.com/CN/abstract/article_29266.shtml} %8 2018-07-01 %X

CRISPR/Cas9 technology has rapidly developed in recent years. It is widely used for study of animals, plants and microorganisms. Among all developments, the use of the technology for the off-target issue has progressed, with important breakthroughs for this hot topic. This paper elucidates the principle of off-target generation and the method for detecting off target both in vitro and in vivo. Also, how to reduce off-targets by improving single-guide RNA design and optimizing the CRISPR system is evaluated. Further more, detecting off-targets at the right time and improving the precision and accuracy of off-target detection are proposed for plant site-specific genome editing.