%A Yuanwei Fan;Aizhi Liu;Huafang Wang
%T Transformation of Populus euphratica
%0 Journal Article
%D 2009
%J Chinese Bulletin of Botany
%R 10.3969/j.issn.1674-3466.2009.06.011
%P 728-734
%V 44
%N 06
%U {https://www.chinbullbotany.com/CN/abstract/article_2616.shtml}
%8 2009-11-01
%X The uniquearbor tree species Populus euphratica can grow well in arid desert. We established an Agrobacterium based transformation system for this species using a GUS gene as the reporter. Shoots of P. euphratica were regenerated from leaves placed on 1/2 MS supplemented with 1.0 mmol.L-1 6-BA and 5.0 mmol.L-1 NAA, leaves were infected for 15 min in a solution of A. tumefaciens with 0.4-0.6 OD600 and 100 mmol.L-1 AS, and then co-cultured for 2 days. A. tumefaciens was inhibited with 300 mg.L-1 Cef, and transgenic tissues and shoots were selected with 9 mg.L-1 G418.