Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (5): 620-624.doi: 10.11983/CBB19127

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana

Xu Wanyue,Wang Yingxiang()   

  1. School of Life Sciences, Fudan University, Shanghai 200438, China
  • Received:2019-07-10 Accepted:2019-07-31 Online:2020-03-10 Published:2019-09-01
  • Contact: Wang Yingxiang E-mail:yx_wang@fudan.edu.cn

Abstract:

Meiosis halves the number of chromosomes to produce haploid gametes through nuclear division twice following single round of DNA replication, and is essential for eukaryotic sexual reproduction. Arabidopsis thaliana is a traditional model organism used for molecular genetic study. Recently, with breakthroughs in microscopic technologies for observing chromosomes, analyses of chromosome morphology during meiosis using cytological methods have made great advances to understand molecular and genetic mechanisms in regulation of meiosis. In this study, we described in detail the chromosome spread technique we developed, which is well used for observation of chromosome behaviors in Arabidopsis male meiocytes.

Key words: Arabidopsis thaliana, meiocytes, chromosome spread, FISH

Figure 1

Chromosome morphology of the male meiocytes of Arabidopsis thaliana Col-0 ecotype (DAPI staining) (A) Leptotene; (B) Zygotene; (C) Pachytene; (D) Diplotene; (E) Diakinesis; (F) Metaphase I; (G) Anaphase I; (H) Telophase I; (I) Prophase II; (J) Metaphase II; (K) Anaphase II; (L) Tetrads. Bars=5 μm"

Figure 2

Chromosome morphology and centromere signals of male meiocytes of the Arabidopsis thaliana Col-0 ecotype (FISH) (A)-(L) see Figure 1. Bars=5 μm"

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