Chinese Bulletin of Botany ›› 2014, Vol. 49 ›› Issue (2): 183-189.DOI: 10.3724/SP.J.1259.2014.00183

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Transformation of RNAi Vector in Cucumber (Cucumis sativus) In Vitro by Agrobacterium tumefaciens-mediated Transfection

Ye Wang, Xingfang Gu*, Shengping Zhang, Han Miao, Guohua Chen, Bingyan Xie   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-26 Revised:2013-04-12 Online:2014-03-01 Published:2014-01-07
  • Contact: Xingfang Gu

Abstract: The RNAi vector of the target gene was transfected into cucumber (Cucumis sativus) cotyledons and cotyledon nodes by Agrobacterium tumefaciens-mediated transfection. A total of 99 regeneration plants were hygromycin-resistant. PCR and Southern-blot analysis revealed that target gene was integrated into the genome of 10 regenerated plants. The transformation efficiency in the cucumber cotyledon and cotyledon node was significantly increased from 4.1% to 13.9% and 3.1% to 6.9%, respectively, when using different hygromycin concentrations in the selection cultivation. We have optimized the genetic transformation system with an RNA interference vector in cucumber and successfully obtained transgenic cucumber plants.