Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (1): 77-88.DOI: 10.11983/CBB16192

Special Issue: 水稻生物学专辑 (2017年52卷1期)

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Identification of Salt Tolerance in Different Rice Germplasm at Different Growth Stages

Jiafu Wu, Bowen Yang, Xunchao Xiang*, Liang Xu, Limei Yan   

  1. Laboratory of Plant Molecular Genetics and Breeding, Southwest University of Science and Technology, Mianyang 621010, China
  • Received:2016-10-07 Accepted:2016-12-13 Online:2017-01-01 Published:2017-01-23
  • Contact: Xiang Xunchao
  • About author:

    # Co-first authors

Abstract: We used 21 rice (Oryza sativa) germplasm as experimental materials. Dry seeds were treated for 8 d in 1.5% NaCl solution and germination rate was measured. The seedlings with 2 green leaves were cultured by hydroponics in Petri dishes with different NaCl concentrations. After 10 days, relative shoot height, relative root length, leaf mortality, water content and the expression of 7 of 9 high-affinity K+ transporter (HKT) gene families were measured. In the adult period (30-d seedling to maturation), 3 representative germplasm were treated at different NaCl concentrations in a pot experiment. We investigated the effects of salt stress at flowering or dough stage of rice, for example, soluble sugar content. The results showed that germination rates of seed were affected significantly, with a wide range of variation, with 1.5% NaCl stress concentration. The variation in leaf mortality was the largest among the measured indicators at seedling stage. Besides OsHKT2;4, 6 members of HKT gene family were detected in the 8 germplasm genome. Soluble sugar content of germplasm showed a growth-stimulation effect with salt content stress ≤1 g∙kg-1. The biomass of CG15R was positively correlated with salt content and the biomass increased with the addition of salt. Biomass of Zhonghua9 increased with the addition of salt with salt content ≤1 g∙kg-1. Salt tolerance of rice shows the characteristic of stage development, and salt tolerance at different developmental stages is not correlated. The leaf mortality and biomass of dough stage could be the key indicators of salt tolerance for seedling and adult rice. CG15R represents a germplasm with high salt tolerance and should be further analyzed.