Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (5): 684-690.DOI: 10.11983/CBB16008

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A Rapid Propagation System for Scirpus × mariqueter

Qun Zhang1,2†, Xiuli Lü1†, Xiaoli He1, Yi Zhu1, Xinhong Cui1*   

  1. 1Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
    2Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Sciences, Fudan University, Shanghai 200438
  • Received:2016-01-13 Accepted:2016-05-12 Online:2016-09-01 Published:2016-09-27
  • Contact: Zhang Qun,Lü Xiuli,Cui Xinhong
  • About author:

    # Co-first authors

Abstract: The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L-1 6-BA+0.002 mg·L-1 TDZ+0.2 mg·L-1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L-1 6-BA+0.01 mg·L-1 IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L-1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.