Chinese Bulletin of Botany ›› 2013, Vol. 48 ›› Issue (1): 59-64.DOI: 10.3724/SP.J.1259.2013.00059

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Rapid Screening of Cry1Ab/c Transgenic Maize Using an Anthocyanin Visualizing Track System

Chaojie Ba1,2†, Jing Xue1†, Xuqing Chen1, Fengping Yang1, Liquan Zhang1, Xianglong Li1, Xiaodong Zhang1*   

  1. 1Beijing Research Center for Agricultural Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;

    2College of Life Sciences, Capital Normal University, Beijing 100037, China
  • Received:2012-02-09 Revised:2012-06-28 Online:2013-01-01 Published:2012-11-01
  • Contact: Xiaodong Zhang

Abstract: Bi and Cl genes, encoding anthocyanin synthesis of transcription factor, were used as visual selection reporter genes, and the glyphosate-resistance gene EPSPS as a selective marker, to generate a visualizing track vector pBAC9017. We used this vector to transform Cry1Ab/c driven by an enhanced CaMV 35S (E35S) promotor, into immature embryos and embryonic callus of maize inbred line 501 by microprojectile bombardment. We obtained 147 transformed, and among 106 plants with harvested T0 ears, 16 ears were decorated with mosaic purple seeds. PCR analysis showed that the Cry1Ab/c gene was integrated into the genome of maize. Extracts of seed protein were tested by BT-Cry1Ab/1Ac immune-strip and ELISA, showing that Bt-Cry1Ab/c gene was expressed in transgenic lines.