Chin Bull Bot ›› 2015, Vol. 50 ›› Issue (5): 565-572.doi: 10.11983/CBB14141

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Radiosensitivity of Green Globular Bodies of Matteuccia struthiopteris Exposed to 60Coγ Radiation

Rongpei Yu1,2, Yang Li1, Dong Li1, Xuanhuai Zhan3, Lei Shi1*   

  1. 1The Key Laboratory of Northern Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

    2National Engineering Research Center for Ornamental Horticulture, Flower Research Institute, Yunnan Academy of Agricultural Science, Kunming 650205, China;

    3Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900, China
  • Received:2014-08-06 Revised:2014-12-14 Online:2015-10-09 Published:2015-09-01
  • Contact: Lei Shi E-mail:shilei@ibcas.ac.cn

Abstract:

Combining tissue culture with radiation mutation techniques, we investigated the radiosensitivity of green globular bodies (GGB) of Matteuccia struthiopteris by exposing it to various doses of 60Coγ radiation. The significant linearity between GGB survival and doses of 60Coγ could be expressed as y=–0.797x+104.719, LD50=69 Gy. The multiplication and differentiation of GGB was decreased and the differentiation period was prolonged with increasing radiation doses. Phenotypic damage such as dark color, death of single green bodies, and cellular damage such as micronuclei, broken nuclei and decrease of embryonic cell after irradiation.

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