Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (5): 569-574.doi: 10.3724/SP.J.1259.2011.00569

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Callus Induction and Plant Regeneration of Rose

Xue Chen, Jinzhu Zhang, Bingbing Pan, Chengjin Sang, Xue Ma, Tao Yang, Daidi Che*   

  1. Horticulture College of Northeast Agricultural University, Harbin 150030, China
  • Received:2011-05-09 Revised:2011-07-05 Online:2011-09-01 Published:2011-09-01
  • Contact: Daidi Che E-mail:daidiche@yahoo.com.cn

Abstract: We used the leaves and stems of aseptic seedlings of 5 rose cultivars that can successfully live outside through the winter in Heilongjiang Province as explants to investigate callus induction and plant regeneration. The leaves and stems of the 5 rose cultivars could induce callus; media containing 2,4-D could induce callus better; and a high concentration of cytokinin was not suitable for callus induction. TDZ played an important role in callus differentiation; culture under light conditions was effective for callus differentiation; and callus differentiation decreased with increasing number of subcultures. Plant regeneration could be successfully induced from the callus of 2004-8 and 2004-9 in experimentation, and the callus differentiation rate of the 2 varieties was 45% and 38%, respectively.

Dohm A., Ludwig C, Nehring K, Debener T(2001).Somatic embryogenesis in roses. Acta Hort (547), 341-346. (期刊中文献)
Hsia C, Korban SS(1996). Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima . Plant Cell Tissue Organ Cult (44),1- 6 (期刊中文献)
Arene L, Pellegrino C, Gudin SA(1993). Comparison of the somaclonal variation level of Rosa hybrida L cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues . Euphytica (71),83 -90. (期刊中文献)
Noriega C, Sondahl MR(1991). Somatic embryogenesis in hybrid tea roses . Biotechnology (9),991-993. (期刊中文献)
李青,苏雪痕,李湛东(1999).藤本月季组织培养快繁研究.北京林业大学学报 21 (6),17~21. (期刊中文献)
李海燕,胡国富,胡宝忠(2004).月季的组培快繁技术的研究.东北农业大学学报 35 (1), 84-88. (期刊中文献)
高莉萍,包满珠(2005). 月季‘萨蔓莎’愈伤组织的诱导及植株再生.园艺学报32(3),534-536.(期刊中文献)
任桂芳, 王建红, 冯慧, 李毅, 李燕, 施雪波(2004).现代月季(Rosa hybrida)叶片植株再生体系的建立. 园艺学报 31(4), 533 - 536. (期刊中文献)
Kintzios S., Manos C., and Makri O(1999). Somatic embryogenesis from mature leaves of rose (Rosa sp.). Plant Cell Rep 18(6), 467-472. (期刊中文献)
Li X., Krasnyanski S.F., and Korban S.S(2002). Somatic embryogenesis, secondary somatic embryogenesis and shoot organogensis in rosa. J. Plant physiol 159(3), 313-319. (期刊中文献)
Rout G R, Debata B K, Das P(1991).Somatic embryogenesis in callus cultures of Rosa hybridaL. cv. Landora. PlantCell Tissue and Organ Culture 27,65~69. (期刊中文献)
Kintzios S., Drossopoulos J B, Lymperopoulos C( 2000). Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose. Journal of Plant Nutrition 23 (10),1407~1420. (期刊中文献)
林娅,郑玉梅,刘青林(2006). 影响月季愈伤组织诱导和分化的因素.分子植物育种 4(2), 223-227.(期刊中文献)
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