Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (4): 486-496.DOI: 10.11983/CBB18139

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Expression Analysis of Key Genes in Gibberellin Biosynthesis and Related Phytohormonal Dynamics During Sugarcane Internode Elongation

Yegeng Fan1,Lihang Qiu1,Xing Huang1,Huiwen Zhou1,Chongkun Gan2,Yangrui Li1,Rongzhong Yang1,Jianming Wu1,*(),Rongfa Chen1,*()   

  1. 1 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement, Ministry of Agriculture/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences, Nanning 530007, China
    2 Chongzuo Jiangzhou Soil Fertility Station, Chongzuo 532200, China
  • Received:2018-06-19 Accepted:2018-12-10 Online:2019-07-10 Published:2020-01-08
  • Contact: Jianming Wu,Rongfa Chen

Abstract: In this study, Saccharum officinarum GT42 was used as the research material. Second internode tissue of sugarcane at the non-elongation stage (Ls1), early elongation stage (Ls2) and elongation stage (Ls3) was collected to measure plant hormones such as gibberellic acid (GA), indole acetic acid (IAA), brassinosteroids (BR), cytokinin (CTK), ethylene (ETH) and abscisic acid (ABA). The differential expression of the key genes GA20-Oxidase1, GID1 and GAI was analyzed by qRT-PCR. During the elongation stage, the contents of GA and IAA showed an upward trend, but CTK and ABA contents showed a downward trend. ETH content was increased at first and then decreased, whereas BR content did not change. The expression of GA20-Oxidase 1 and GID1 increased, and that of GAI decreased, which was closely related to the changes in GA content. Therefore, we considered that the internode elongation of sugarcane is mainly related to GA and IAA, then are CTK and ABA, and ETH is regulated by IAA to affect internode elongation. The interaction between these phytohormones regulates the expression of GA20-Oxidase 1, GID1 and GAI, which affects the content and signal transduction of GA, thereby affecting the internode elongation of sugarcane. The study clarifies the differential expression of key genes in the gibberellin biosynthesis pathway and signal transduction process, along with the dynamic changes of plant hormone content during internode elongation of sugarcane.

Key words: phytohormone, gibberellin, gene expression analysis, internode elongation, sugarcane