Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (5): 612-624.DOI: 10.11983/CBB17117

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Genome-wide Identification of Ethylene Responsive Factor (ERF) Family Genes in Peach and Screening of Genes Related to Germination

Zhao Xuehui1,2, Wang Qingjie1,2, Li Chen1,2, Chen Xiude1,2, Xiao Wei1,2, Gao Dongsheng1,2, Fu Xiling1,2,*()   

  1. 1College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;
    2State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China;
  • Received:2017-06-15 Accepted:2017-10-16 Online:2018-09-01 Published:2018-11-29
  • Contact: Fu Xiling
  • About author:

    † These authors contributed equally to this paper

Abstract: In this study, genome-wide identification of ERF family genes in ‘zhongyousihao’ peach was studied by using MEGA 6.0, MEME, GSDS, DNAMAN 6.0. We identified 102 genes, and phylogenetic analyses of the genes indicated that the transcription factors can be classified into 10 different groups (I-X). Gene structure analysis showed that 81 genes had no introns, 20 genes contained an intron, and 1 gene contained 5 introns, which had a large difference from other members. Conservative motif analysis showed that the ERF family contained 20 conserved elements: Motifs 1, 2 and 4 belonged to the APETALA2 (AP2)/ERF domain, and the same conserved motif appeared mainly in the same family; the function of most conserved motifs is unknown. qRT-PCR revealed that the expression of PpeERF068 during dormancy and germination was consistent with the developmental status of leaf buds, which suggested that it might be related to leaf bud germination. The gene expression of peach buds during each period in light culture incubation further showed the relation between PpeERF068 and the germination of leaf buds. So PpeERF068 was named PpeEBB1. We will further study the molecular mechanism of PpeEBB1 to provide theoretical guidance for the cultivation and management of peach.

Key words: peach, ERF family, bud germination, PpeEBB1