Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (5): 506-513.doi: 10.3724/SP.J.1259.2011.00506

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Characterization and Gene Mapping of a Novel Mutant in Rice Floral Organs

Weixiong Luo1, Ming Li2, Jun Chen1, Qiong Luo1*   

  1. 1Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Ministry of Education/Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China;
    2State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2011-01-27 Revised:2011-04-25 Online:2018-12-06 Published:2011-09-01
  • Contact: Qiong Luo E-mail:qiongbf@yahoo.com.cn

Abstract: We obtained a rice floral mutant, inner flower organ number 6 (fon6), from the progenies of tissue culture of a japonica variety Taichung65. The mutant has double ovaries, multi-stigmas, and 7 to 8 stamens. The mutant trait is controlled by a single recessive gene. The F2 population derived from the cross between Zhongxian 3037 and fon6 was used for gene mapping. FON6 was mapped to the ~480-kb region between the sequence-tagged site markers PL4 and PL5 on chromosome 6. These results will be useful for cloning and functional analysis of the gene.

Key words: double-ovary, flower organ, gene mapping, multi-stigmas, rice

计慎敏, 张大兵 (2007). 水稻花器官特征决定以及数量控制的分子机理. 植物学通报 24, 284–292.
张向前, 邹金, 朱海涛, 李晓燕, 曾瑞珍 (2008). 水稻早熟突变体fon5的遗传分析和基因定位.遗传30, 1349–1355.
Agrawal GK, Abe K, Yamazaki M, Miyao A and Hirochika H (2005). Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene. Plant Mol Bio 59, 125–135.
Arora R, Agarwal P, Ray S, Singh AK, Singh VP, Tyagi AK and Kapoor S (2007). MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress. BMC Genomics 8, 242.
Chu HW, Qian Q, Liang WQ, Yin CS, Tan HX, Yao X, Yuan Z, Yang J, Huang H, Luo D, Ma H and Zhang DB (2006).The FLORAL ORGAN NUMBER4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice. Plant Physiology 142 1039–1052.
Clark SE, Running MP, Meyerowitz EM (1993). CLAVATA1, a regulator of meristem and flower development in Arabidopsis. Development 119, 397-418.
Coen ES, Meyewitz EM (1991). The war of the whorls: genetic interactions controlling flower development. Natural 353, 31–37.
Coen ES, Romero JM, Doyle S, Elliott R, Murphy G and Carpenter R (1990). floricaula: A homeotic gene required for flower development in Antirrhinum majus. Cell 63, 1311–1322.
Colombo L, Franken J, Koetje E, Van Went J, Dons H, Angenent G and Van Tunen A (1995). The petunia MADS box gene FBP11 determines ovule identity. Plant Cell 7,1859–1868.
Fletcher JC, Brand U, Running MP, Simon R, Meyerowitz EM (1999). Signaling of cell fate decisions by CLAVATA3 in Arabidopsis shoot meristems. Science 283, 1911-1914.
Fornara F, Marziani GA, Mizzi L, Kater M and Colombo L (2003). MADS-box genes controlling flower development in rice. Plant Boil 5, 16–22.
Goto K, Meyerowitz EM (1994). Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA. Genes Dev 8, 1548–1560.
Hong LL, Qian Q, Zhu KM, Tang D, Huang ZJ, Gao L, Li M, Gu MH and Cheng ZK (2010). ELE restrains empty glumes from developing into lemmas. J Genet Genomics 37, 101–115.
Jack T, Brochman LL, and Meyerowitz EM (1992). The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell 68, 683–697.
Jeon J, Lee S, Jung K, Yang W, Yi G, Oh B and An G (2000). Production of transgenic rice plants showing reduced heading date and plant height by ectopic expression of rice MADS-box genes. Mol Breed 6, 581–592.
Jiang L, Qian Q, Mao L, Zhou QY and Zhai WX (2005). Characterization of the rice floral organ number mutant fon3. Journal of Integrative Plant Biology 47, 100?106.
Lee S, Kim J, Son JS, Nam J, Jeong DH, Lee K, Jang S, Yoo J, Lee J, Lee DY, Kang HG, An G (2003). Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case. Plant Cell Physiol 44, 1403-1411.
Li HF, Liang WQ, Jia RD, Yin CS, Zong J and Zhang DB (2010). The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice. Cell Research 20, 299–313.
Li Y, Xu PZ, Zhang HY, Peng H, Zhang QF, Wang XD and Wu XJ (2007). Characterization and identification of a novel mutant fon(t) on floral organ number and floral organ identity in rice. J Genet Genomics 34, 730–737.
Lim J, Moon YH, An G and Jang S K (2000). Two rice MADS domain proteins interact with OsMADS1. Plant Mol Biol 44, 513–527.
Mandel MA, Gustafson-Brown C, Savidge B, and Yanofsky M (1992). Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature 360, 273–277.
Moon Y, Kang H, Jung J, Jeon J, Sung S and An G(1999). Determination of the motif responsible for interaction between the rice APETALA1/AGAMOUS-LIKE9 family proteins using a yeast two-hybrid system. Plant Physiology 120, 1193–1204.
Murray MG, Thompson WF (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8, 4321–4326.
Nagasawa N, Miyoshi M, Sano Y, Satoh H, Sakai H and Nagato Y (2003). SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development 130, 705–718.
Nam J, Kim J, Lee S, An G, Ma H and Nei M (2004). Type I MADS-box genes have experienced faster birth-and death evolution than II MADS-box genes in angiosperms. PNAS 101, 1910-1915.
Pelaz S, Ditta G, Baumann E, Wisman E and Yanofsky M (2000). B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405, 200–203.
Sommer SZ, Huijser P, Nacken W, Saedler H and Sommer H (1990). Genetic control of flower development by homeotic genes in Antirrhinum majus. Science 250, 931–936.
Sun QW, Zhou DX (2008). Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development. PNAS 105, 13679–13684.
Suzaki T, Ohneda M, Toriba T, Yoshida A and Hirano HY (2009). FON2 SPARE1 redundantly regulates floral meristem maintenance with FLORAL ORGAN NUMBER2 in Rice. Plos Genetics 5, e1000693.
Suzaki T, Sato M, Ashikari M, Nagato Y and Hirano HY (2004). The gene FLORAL ORGAN NUMBER1 regulates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development 131, 5649?5657.
Theissen G, Saedler H (2001). Floral quartets. Nature 409, 469–471.
Weigel D, Meyerowitz EM (1994). The ABCS of floral homeotic genes. Cell 78, 203–209.
Yamaguchi T, Hirano HY (2006). Function and diversification of MADS-box genes in rice. TSW Development & Embryology 1, 99–108.
Yanofsky M, Ma H, Bowman J, Drews G, Feldmann K and Meyerowitz E (1990). The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature 346, 35–39.
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