Chinese Bulletin of Botany ›› 2011, Vol. 46 ›› Issue (3): 293-301.DOI: 10.3724/SP.J.1259.2011.00293

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Breeding and Salt Resistance Evaluation of BADH Transgenic Alfalfa Cultivar Shanmu 2

Liping Yan1,2, Yang Xia1,2*, Xiuhong Mao1,2, Cuilan Liu1,2, Huimin Liang3   

  1. 1Shandong Provincial Academy of Forestry, Jinan 250014, China;

    2Shandong Provincial Key Laboratory of Forest Tree GeneticImprovement, Jinan 250014, China;

    3Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China
  • Received:2010-10-05 Revised:2011-01-31 Online:2011-05-01 Published:2011-05-26
  • Contact: Yang Xia

Abstract: Generating new germplasm of alfalfa by transgenic technology has become important in forage breeding. Using a T0 generation of transgenic plants with betaine aldehyde dehydrogenase (BADH) gene as testing material, we tested the resistance to salt. The transgenic grass biomass was higher than that of the non-transgenic control in different salt soils. Dry grass production was higher by 13.11% to 24.98% in the transgenic cultivar than in the control. The betaine aldehyde dehydrogenase gene was stably expressed and inherited, suggesting that this transgenic line can be used for further breeding.

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