Chin Bull Bot ›› 2010, Vol. 45 ›› Issue (03): 337-344.doi: 10.3969/j.issn.1674-3466.2010.03.005

• 研究报告 • Previous Articles     Next Articles

Ultracytochemical Localization of Ca2+ and Ca2+-ATPase in the Root of Puccinellia tenuiflora Under NaHCO3 Stress

Lingling Fan1; Gang Chen3; Yifang Chen3; Weidong Zhou3; Shaojun Dai1*; Guorong Sun2*   

  1. 1Northeast Forestry University, Harbin 150040, China;
    2Binzhou Polytechnic College, Binzhou 256603, China;
    3Yangzhou University, Yangzhou 225009, China
  • Received:2009-06-16 Revised:2009-11-18 Online:2010-05-01 Published:2010-03-01
  • Contact: Shaojun Dai;Guorong Sun

Abstract: We aimed to further investigate the relationships between the localization of calcium and Ca2+-ATPase and the resistance capacity of Puccinellia tenuiflora against saline-alkali stress. We analyzed ultracytochemical localization of calcium and Ca2+-ATPase in the root of P. tenuiflora under NaHCO3 stress by potassium pyroantimonate precipitation and a lead-phosphate precipitation technique, respectively. In the normal condition, calcium antimonate precipitation deposits were low in cytoplasm and mainly concentrated within the plasma membrane and vacuoles in the root hair zone. Ca2+-ATPase had a certain activity and mainly localized in the plasma membrane and tonoplasts. Moreover, under 0.448% NaHCO3 stress, the distribution of Ca2+ precipitation particles increased in the cytoplasm, decreased in the vacuole, and mainly concentrated near tonoplasts. Meanwhile, the activity of Ca2+-ATPase was significantly increased in the plasma membrane and tonoplasts. Furthermore, under 1.054% NaHCO3, Ca2+ precipitation particles continued to increase in the cytoplasm but were absent in the vacuole. The activity of Ca2+-ATPase was also reduced. All these results highlight that the changes in Ca2+ localization and Ca2+-ATPase activity play important roles in P. tenuiflora resistance to saline-alkali stress.

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