Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (1): 58-63.doi: 10.11983/CBB17237

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Genetic Diversity and Population Structure of Eggplant (Solanum melongena) Germplasm Resources Based on SRAP Method

Xie Lifeng1,Li Ning2,*(),Li Ye1,*(),Yao Minghua2   

  1. 1 Harbin Academy of Agricultural Sciences, Harbin 150028, China
    2 Institute of Economic Crops of Hubei Academy of Agricultural Sciences, Wuhan 430064, China
  • Received:2017-12-06 Accepted:2018-08-21 Online:2019-07-31 Published:2019-01-01
  • Contact: Li Ning,Li Ye E-mail:n.li@msn.com;eggplant_2010@163.com

Abstract:

The genetic diversity and the population structure of 183 germplasm resources of eggplant were analyzed by using SRAP markers. Overall, 215 bands were generated with 7 binds per primer on average. The mean genetic similarity coefficient was 0.623 among the cultivars (0.276-0.813), which suggested genetic differentiation among the germplasm resources. The germplasm resources were divided into four groups with a similarity coefficient of 0.345 6. Introgression exists in the four groups based on population structure analysis. The boundaries among different groups are obvious, and gene introgression among varieties is high.

Key words: eggplant, genetic diversity, population structure, SRAP analysis

Table 1

The results of polymorphic amplification of 33 pairs of primer combination"

Primer combinations Allele frequencies Number of polymorphic bands Genetic
diversity
PIC
Me1Em3 0.6721 4 0.4962 0.4486
Me1Em4 0.5519 4 0.5896 0.5215
Me1Em7 0.8798 4 0.2210 0.2133
Me1Em8 0.5792 5 0.5838 0.5283
Me1Em9 0.7432 4 0.4176 0.3839
Me2Em3 0.2732 11 0.8556 0.8413
Me2Em4 0.5246 7 0.6770 0.6492
Me2Em5 0.3525 13 0.7619 0.7295
Me2Em6 0.6393 6 0.5606 0.5349
Me3Em1 0.7268 6 0.4511 0.4289
Me3Em2 0.3060 8 0.7718 0.7368
Me3Em3 0.3060 8 0.7823 0.7509
Me5Em1 0.4973 4 0.6209 0.5525
Me5Em2 0.3333 12 0.7877 0.7598
Me5Em7 0.3607 7 0.7106 0.6571
Me5Em8 0.4208 4 0.6791 0.6205
Me5Em9 0.4754 6 0.6854 0.6412
Me5Em11 0.6120 5 0.5774 0.5400
Me6Em7 0.4262 8 0.7518 0.7247
Me6Em8 0.4590 6 0.6636 0.6035
Me6Em9 0.5738 5 0.6127 0.5723
Me6Em11 0.7268 6 0.4363 0.3987
Me7Em3 0.6557 5 0.4901 0.4211
Me7Em5 0.7486 7 0.4237 0.4058
Me7Em8 0.6557 5 0.5184 0.4733
Me7Em9 0.3224 7 0.7684 0.7338
Me7Em11 0.5464 5 0.5994 0.5368
Me7Em12 0.7486 7 0.4210 0.4001
Me9Em1 0.6011 5 0.5344 0.4582
Me9Em3 0.6284 6 0.5377 0.4839
Me9Em13 0.6667 8 0.5197 0.4874
Me8Em6 0.6885 12 0.5055 0.4860
Me8Em7 0.5410 5 0.5252 0.4185
Mean 0.5528 7 0.5920 0.5498

Figure 1

SRAP amplification of primer Me1Em8 in some eggplant varieties (The number of 1-46 see Appendix)"

Figure 2

Cluster analysis for 183 eggplant varieties (See Appendix for the detailed information)"

Figure 3

Population structure of 183 eggplant varieties"

1 冯英娜, 柳李旺, 刘卫东, 王倩, 崔群香 ( 2014). 茄子SSR遗传多样性及其农艺性状的关联分析. 江苏农业学报 30, 839-847.
doi: 10.3969/j.issn.1000-4440.2014.04.024
2 何倚剑, 刘卫东, 柳李旺, 王倩, 崔群香 ( 2013). 茄子种质资源遗传多样性和群体结构分析. 南京农业大学学报 36(5), 13-20.
doi: 10.7685/j.issn.1000-2030.2013.05.003
3 李宁, 姚明华, 焦春海, 李烨, 王飞 ( 2014). 亚洲及非洲茄子种质资源主要农艺性状的遗传多样性分析. 湖北农业科学 53, 5769-5774.
doi: 10.14088/j.cnki.issn0439-8114.2014.23.037
4 李涛, 黎振兴, 罗少波, 李植良, 徐小万 ( 2014). 东南亚茄子种质资源ISSR遗传多样性分析. 中国农学通报 30(25), 104-110.
5 刘丽华, 王立新, 赵昌平, 姚骥, 张风廷, 张华, 叶志杰, 秦志列, 郑用琏 ( 2009). 光温敏二系杂交小麦恢复系遗传多样性和群体结构分析. 中国生物化学与分子生物学报 25, 867-875.
6 毛伟海, 杜黎明, 包崇来, 胡天华, 朱琴妹, 胡海娇 ( 2006). 我国南方长茄种质资源的ISSR标记分析. 园艺学报 33, 1109-1112.
doi: 10.3321/j.issn:0513-353X.2006.05.040
7 冉进, 宋明, 房超, 文明玲, 刘小俊, 刘独臣, 李跃建 ( 2007). 茄子(S. melongena L.)种质资源遗传多样性的RAPD分析. 西南农业学报 20, 694-697.
doi: 10.3969/j.issn.1001-4829.2007.04.029
8 孙源文, 陈钰辉, 刘富中, 张映, 连勇 ( 2012). 基于SSR分子标记的栽培种茄子遗传多样性分析. 中国蔬菜 ( 22), 17-23.
9 王秋锦, 高杰, 孙清鹏, 杨爱珍, 赵福宽 ( 2007). 茄子品种遗传多样性的RAPD检测与聚类分析. 植物生理学报 43, 1035-1039.
10 An CF, Saha S, Jenkins JN, Ma DP, Scheffler BE, Kohel RJ, Yu JZ, Stelly DM ( 2008). Cotton ( Gossypium spp.) R2R3-MYB transcription factors SNP identification, phylogenomic characterization, chromosome localization, and linkage mapping.Theor Appl Genet 116, 1015-1026.
doi: 10.1007/s00122-008-0732-4 pmid: 18338155
11 Falush D, Stephens M, Pritchard JK ( 2007). Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol Ecol Notes 7, 574-578.
doi: 10.1111/j.1471-8286.2007.01758.x pmid: 18784791
12 Flint-Garcia SA, Thornsberry JM, Buckler ES ( 2003). Structure of linkage disequilibrium in plants. Annu Rev Plant Biol 54, 357-374.
doi: 10.1146/annurev.arplant.54.031902.134907 pmid: 14502995
13 Li G, Quiros CF ( 2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica.Theor Appl Genet 103, 455-461.
doi: 10.1007/s001220100570
14 Li HZ, Chen HY, Zhuang TM, Chen J ( 2010). Analysis of genetic variation in eggplant and related Solanum species using sequence-related amplified polymorphism markers.Sci Hortic 125, 19-24.
doi: 10.1016/j.scienta.2010.02.023
15 Perrier X, Flori A, Bonnot F (2003). Data analysis methods. In: Hamon P, Seguin M, Perrier X, eds. Genetic Diversity of Cultivated Tropical Plants. Montpellier: Enfield, Science Publishers. pp. 43-76.
16 Rohlf FJ ( 1997). NTSYS-pc-Numerical Taxonomy and Multivariate Analysis System Version 2.02e. Exeter Software. New York.
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