Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (1): 43-53.DOI: 10.11983/CBB16081

Special Issue: 水稻生物学专辑 (2017年52卷1期)

• Orginal Article • Previous Articles     Next Articles

Molecular Evolution and Expression Analysis of the OsMIP1 Response to Abiotic Stress

Wang Ling1,2, Guo Changkui1,2,3, Ren Ding1,2,*(), Ma Hong1,2,*()   

  1. 1School of Life Sciences, Fudan University, Shanghai 200438, China
    2Institute of Plant Biology, Fudan University, Shanghai 200438, China
    3School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
  • Received:2016-04-16 Accepted:2016-05-26 Online:2017-01-01 Published:2017-01-23
  • Contact: Ren Ding,Ma Hong
  • About author:

    # Co-first authors

Abstract: The gene MID1 (MYB IMPORTANT FOR DROUGHT RESPONSE1) encodes a putative R-R type MYB transcription factor; is induced by abiotic stresses, especially drought in reproductive stage; and can improve pollen fertility and rice production. To understand the role of MID1 in abiotic stress responses, we used the yeast two-hybrid system to find an interacting protein, OsMIP1 (Oryza sativa MID1 interaction protein 1). The interaction was further confirmed by BiFC (bimolecular fluorescence complementation) analysis in tobacco leaf cells. OsMIP1 encodes a putative transmembrane protein with an ENTH/ANTH/VHS domain. It is expressed in the root, stem, leaf, panicle and endosperm. Under drought stress, its expression is upregulated in leaf and reproductive organs, especially in post-meiotic flowers. OsMIP1 may play a role in response to drought stress during reproductive development. OsMIP1 expression during vegetative development can be induced by other abiotic stress, including NaCl and mannitol, which suggests that OsMIP1 can respond to other abiotic stresses. There is little analysis of the evolution of genes encoding proteins with the ENTH/ ANTH/VHS domain, so we analyzed the molecular evolution of MIP1 homologs in flowering plants. The evolution analysis of the MIP1 family in angiosperms showed that MIP1 homologs can be divided into 6 types, which originated from at least 6 copies of MIP1 homologous genes in the ancestor of extent angiosperms. After gene-duplication and -loss events, MIP1 family members widely distributed in the angiosperms and might have various functions, possibly in stress responses.