Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (1): 58-67.doi: 10.11983/CBB14211

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Chemical Changes Caused by LED Lamps in Nutrient Solution and Its Effect on Plant Growth

Jia Li, Jinxing Chen, Zhizheng Li*   

  1. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
  • Online:2016-02-01 Published:2016-01-01
  • Contact: Li Zhizheng E-mail:zzl@sippe.ac.cn
  • About author:? These authors contributed equally to this paper

Abstract:

Rice plants were grown in nutrient solution containing Si/FeEDTA and LED light. Plants grown distant from the light source could get effect carried by the circulation of nutrient solution. The solution containing Fe (100 mg∙L-1, FeEDTA) and Si (100 mg∙L-1, K2SiO3) was illuminated separately by sunlight and LED-purple in the solution. The OD400 of the solution increased with each illuminating day. The chemical change induced by illumination occurred between FeEDTA and K2SiO3. This chemical change was not related to solution temperature (°C) but was related to illumination only, so this was a photochemical reaction. The illuminated solution resulted in light absorbance from OD360 to OD560. An FeEDTA-SiO3 complex was isolated from the solution illuminated by LED blue or sunlight. This complex was again absorbed by a lining membrane to form a three-component complex. There was a large amount of complex (e.g., microcrystals) accumulated on the lining membrane. LED-purple, LED-red and LED-infrared could be induced to result in the FeEDTA-SiO3 complex. Chlorella beijerinch in the nutrient solution containing the FeEDTA-SiO3 complex grew well; dead algae broke down and left a brown-coloured fluffy ball of Fe-Si complex. The rice plants fed the FeEDTA- SiO3 complex showed high dry weight per plant.

Key words: LED, sunlight, photochemical reaction, FeEDTA-SiO3 complex, plant growth

Figure 1

6 different LED devices for root system illumination experiments6 kinds of LED: LED-red, LED-green, LED-yellow, LED- purple, LED-blue, and LED-white"

Figure 2

The schematic diagram of vertical section of the culture device"

Figure 3

The Fe deficiency symptom and its recovery of rice plants in series experiments of LED illumination to root system in the solution (A) Fe deficiency symptom of rice plants in solution with 1 mg∙L-1 Fe; (B)-(G) The recovery of Fe deficiency symptom of rice plants in solution with 6 mg∙L-1 Fe; The experimental groups were LED-blue, LED-white, LED-purple, LED-green, LED-yellow and LED-red, respectively; Sets in each group, from left to right, was CK3 soil culture, CK2, independent hydroponics, and CK1/LED illuminated in connected hydroponics. (A)-(D) The LED board was in the right pot; (E)-(G) The LED board was in the left pot."

Table 1

Composition of imitated solution reaction system"

Reaction system (RS) Ingredients Compound and source
RS1 100 mg∙L-1 Fe+100 mg∙L-1 Si FeEDTA (self-made), K2SiO3·xH2O (goods)
RS2 100 mg∙L-1 Fe+100 mg∙L-1 Si FeEDTA (Librel BMX), K2SiO3·xH2O (goods)

Figure 4

The OD400 changes induced by sunlight and LED- purple in system RS1"

Table 2

Effects of sunlight and water temperature on the system RS1"

Treatment time (d) Sunlight RS1 in dark (CK) Sunlight RS1
OD400 Water (°C) OD400 Water (°C)
0 0.10 - 0.10 -
1 0.10 38 0.12 35
2 0.10 27 0.13 26
3 0.10 26 0.14 25
4 0.10 24 0.15 24
5 0.10 24 0.16 24
6 0.10 30 0.17 30
7 0.10 27 0.18 27
8 0.11 33 0.19 32
9 0.11 33 0.20 32
10 0.11 32 0.23 31

Figure 5

The spectrums of RS2 solution under sunshine for 28 d (A) and analysis of photo-chemical reaction (B) induced by SiO3 in RS2 system"

Figure 6

The absorption spectrum of the photo chemical outcomes induced by different light source in the solution"

Figure 7

Analysis of contents of photo-chemical outcomes(A) Separate peak 1+2 and peak 6 from the RS1 system in the dark; (B) The complex composed from RS1 system under the sunlight; (C) The complex composed from RS1 system under the LED-blue"

Figure 8

Morphological characteristics and chemical constituents of FeEDTA-SiO3 complex(A) The organic membrane with bubbles of the complex of FeEDTA-SiO3 under the electron microscope (Bar=50 nm); (B) The membrane overlap of FeEDTA-SiO3 complex (attention to a lot of pearls on the membrane) (Bar=100 nm); (C) The chemical elements involved in the organic membrane of FeEDTA-SiO3 complex under the electron microscope; (D) The microcrystal pearls on the membrane of FeEDTA-SiO3 complex"

Figure 9

Effect of primary extracts of FeEDTA-SiO3 com- plex on the growth of Chlorella beijerinch(A) From left to right in proper order: 0.5 mg∙L-1 extract with 90% alcohol, 0.5 mg∙L-1 extract with pure alcohol, CK, 0.2 mg∙L-1 extract in the dialysis bag, and 0.4 mg∙L-1 extract out of the dialysis bag; (B) The samples were in the dark box of 40°C for 40 d (from left to right in proper order were same as in Figure 9A)"

Table 3

Effect of the FeEDTA-SiO3 on rice growth"

Source and dosage of extracts Tiller number Root/shoot Dry weight (g)
(1) CK 3.0 0.09±0.02 0.67±0.12
(2) Pure alcohol extracts, out of the dialysis bag, OD400=0.025 4.2 0.15±0.01 1.25±0.02
(3) Ditto, OD400=0.075 4.3 0.17±0.03 1.49±0.32
(4) Ditto, OD400=0.22 2.3 0.08±0.01 0.63±0.10
(5) 95% alcohol extracts, out of the dialysis bag, OD400=0.042 3.8 0.15±0.03 1.29±0.21
(6) Ditto, OD400=0.176 3.6 0.16±0.02 1.42±0.08
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