Chin Bull Bot ›› 2010, Vol. 45 ›› Issue (06): 744-750.doi: 10.3969/j.issn.1674-3466.2010.06.012

Previous Articles     Next Articles

Rapid Propagation in Vitro of Chiritopsis repanda var. guilinensis

Ningzhen Huang*, Chuanming Fu, Zhiguo Zhao, Fengluan Tang, Yunping Shi   

  1. Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Academia Sinica, Guilin 541006, China
  • Received:2010-04-29 Revised:2010-06-17 Online:2010-09-20 Published:2010-11-01
  • Contact: Ningzhen Huang E-mail:hnzhen68@yahoo.com.cn, huangnz@gxib.cn

Abstract: We investigated in vitro culture and rapid propagation of the antituberculosis plant Chiritopsis repanda var. guilinensis. The optimal medium for primary culture for bud induction and seedling regeneration from leaves was MS+0.5 mg·L–16-BA+0.05 mg·L–1IBA (pH8.0). The optimal medium for subculture for bud multiplication was MS+0.1 mg·L–16-BA+0.05 mg·L–1IBA (pH6.0); the proliferation coefficient was 7.0/35 d. The optimal rooting medium was 1/2MS+0.2mg·L–1NAA (pH6.0), and the rooting rate was 93.6%. The rooting plantlets were transplanted in a greenhouse that simulated the natural distribution environment of Chiritopsis repanda var. guilinensis in spring; the survival rate was more than 90%. According to this rapid propagation technique, about 460 000 seedlings were reproduced from a germfree plantlet in a year.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Cui Gao;Yuxia Chen;Ying Bao;Min Feng;Anming Lu. Studies on Sexual Organs and Embryological Development Morphology of Speirantha gardenii (Convallariaceae)[J]. Chin Bull Bot, 2010, 45(06): 705 -712 .
[2] Jiang Gao-ming. The Impact of Globae Increasing of CO2 on Plants[J]. Chin Bull Bot, 1995, 12(04): 1 -7 .
[3] Zhang Jun Han Bi-wen. Advance in the Study of Histochemical Localization for[J]. Chin Bull Bot, 1995, 12(专辑3): 131 -142 .
[4] Tang Yan-cheng. A Short Guide to the International Code of Botanical Nomenclature V.[J]. Chin Bull Bot, 1984, 2(04): 51 -57 .
[5] Xu Ji. The Protective Protein of Nitrogenase Against Oxygen Damage-Fe-S Protein[J]. Chin Bull Bot, 1986, 4(12): 1 -4 .
[6] . [J]. Chin Bull Bot, 2001, 18(05): 633 .
[7] Huang Zhao-xiang;Zheng Zhen-gui and Zhu Du. Ecological Effect of Taxodium ascendens-Oryza sativa Ecosystem(I) The Growing Characteristic of Taxodium Ascendens in the Ecosystem[J]. Chin Bull Bot, 1996, 13(02): 48 -51 .
[8] Zhihong Xu;Jiayang Li. Plant Hormones Research in China: Past, Present and Future[J]. Chin Bull Bot, 2006, 23(5): 433 -442 .
[9] GU Rui-Sheng;LIU Qun-Lu;CHEN Xue-Mei and JIANG Xiang-Ning. Comparison and Optimization of the Methods on Protein Extraction and SDS-PAGE in Woody Plants[J]. Chin Bull Bot, 1999, 16(02): 171 -177 .
[10] Jiang Gao-ming. LI-6400 Portable Photosynthesis System: Principle, Function, Basic Operation and Main Problems and Solutions During Measurement[J]. Chin Bull Bot, 1996, 13(增刊): 72 -76 .