Chinese Bulletin of Botany ›› 2010, Vol. 45 ›› Issue (05): 604-608.DOI: 10.3969/j.issn.1674-3466.2010.05.010

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Callus Induction and Plant Regeneration of a Living Fossil, Metasequoia glyptostroboides

Cui Huang1, Danlong Jing1, Yubing Wang1, Shenhou Fan2, Faju Chen1*   

  1. 1Biotechnology Research Center and Key Laboratory of Plant Natural Products of Hubei, China Three Gorges University,Yichang 443002, China;

    2Hubei Lichuan Metasequoia Mother Trees Administrative Station, Lichuan 445400, China
  • Received:2009-10-09 Revised:2010-03-25 Online:2010-09-01 Published:2010-09-20
  • Contact: Faju Chen

Abstract: We optimized a rapid, effective system of plant regeneration by direct shoot regeneration and organogenesis in an endangered tree, the living fossil Metasequoia glyptostroboides. We studied the influence of plant growth regulators on explant types (embryo, root, stem and young leaf). Callus could be induced by different types of explants on MS medium supplemented with different combinations of 2,4-D, NAA and 6-BA. The frequency of callus induction was highest (100%) with  embryo used as explants, then with stem (97.1%). The best media for callus induction on embryo was MS+1.0 mg·L–1 2,4-D+0.5 mg·L–1 6-BA, MS+0.1 mg·L–1 6-BA+1.0 mg·L–1 NAA, MS+0.5 mg·L–1 6-BA+1.0 mg·L–1 NAA, MS+1.0 mg·L–1 6-BA+1.0 mg·L–1 NAA, MS+0.5 mg·L–1 6-BA+2.0 mg·L–1 NAA, MS+1.0 mg·L–1 6-BA+2.0 mg·L–1 NAA and MS+0.5 mg·L–1 2,4-D+0.5 mg·L–1 NAA. More regenerated plants were obtained on MS medium, with a frequency of 62.5%.