Chin Bull Bot ›› 2010, Vol. 45 ›› Issue (04): 460-465.doi: 10.3969/j.issn.1674-3466.2010.04.009

• 技术方法 • Previous Articles     Next Articles

orting of early developmental non-hair cells in root by flow cytometry in Arabidopsis thaliana

Fei Li&; Yong Hu&; Fan Wang; Zhen Zhang; Xianglin Liu*; Sulan Bai*; Yikun He   

  1. College of Life Sciences, Capital Normal University, Beijing 100048
  • Received:2010-01-18 Online:2010-09-26 Published:2010-04-30
  • Contact: Xianglin Liu;Sulan Bai

Abstract: We have developed a method for sorting specific cells in plant. Here, WER::GFP transgenic lines in Arabidopsis thaliana were as the experimental material; laser scanning confocal microscopy was used to identify the expression location of GFP; enzymatic digestion was applied for preparation of root tip protoplasts; fluorescence activated cell sorting (FACS) was applied to collect GFP positive cells; and then the total RNA was extracted from these cells. The results showed that only the early developmental non-root hair cells in the root epidermis express GFP in Wer::GFP transgenic lines; larger number of root tip protoplasts were obtained by using enzymatic digestion; and then GFP positive cells were collected successfully by FACS. The RNA extracted from these specific cells can be used for gene expression profiling. The method for sorting specific cells had been established successfully.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Gu Li-ping and Zhou Ruan-bao. Advances on the Study of the Role of Jasmanates in Plants[J]. Chin Bull Bot, 1997, 14(01): 31 -35 .
[2] ZHU Jing TIAN Xing-Jun CHEN Bin LV Jin-Zi. Computer Recognition System of Plant Leaf-shape[J]. Chin Bull Bot, 2005, 22(05): 599 -604 .
[3] Shaofeng Li, Xiaohua Su, Bingyu Zhang. Research Progress in Gene Cloning in Forest Trees[J]. Chin Bull Bot, 2011, 46(1): 79 -107 .
[4] . [J]. Chin Bull Bot, 2004, 21(06): 689 -699 .
[5] Yongdong Sun, Weirong Luo, Xinzheng Li, Guangyin Wang. Effect of Exogenous NaHS on Seed Germination and Physiological Characteristics of Cucurbita ficifolia Under NaHCO3 Stress[J]. Chin Bull Bot, 2014, 49(1): 98 -104 .
[6] Li Yong-hong. [J]. Chin Bull Bot, 1993, 10(增刊1): 42 -51 .
[7] WU Hong-Yan ZHAO Yi-JunGUO Hou-Liang ZHANG Ting WU Tao. The Cellular Effect of Anabaena sp.595 Induced by Inorganic Salts[J]. Chin Bull Bot, 2003, 20(02): 205 -211 .
[8] Guiwei Zhang, Jue Zeng, Wei Guo, Qiong Luo. Bioinformatics Analysis of the AT-hook Gene Family in Rice[J]. Chin Bull Bot, 2014, 49(1): 49 -62 .
[9] Zhang Song-lin Jin Zhi-lan. A Simple Method to Prevent the Specimen of Paraffin Section Trom Shedding During Staining[J]. Chin Bull Bot, 1994, 11(02): 61 .
[10] MA Dong-Ming YANG Tai-Xin CHEN Qing-Liang ZHAI Zhi-Xi GUO Yu-Hai. In vitro Inoculation of Cistanche tubulosa Seeds on Tube Seedlings of Tamarix chinensis[J]. Chin Bull Bot, 2005, 22(06): 687 -691 .