Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (03): 317-322.doi: 10.3969/j.issn.1674-3466.2009.03.008

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Variation of DNA Methylation in Arabidopsis thaliana Seedlings After the Cryopreservation

Yanxia He, Zicheng Wang*   

  1. Institute of Agricultural Biotechnology, Henan University, Kaifeng 475004, China
  • Received:2008-08-14 Revised:2008-11-14 Online:2010-11-03 Published:2009-05-01
  • Contact: Zicheng Wang

Abstract:

We analyzed the variation patterns of DNA methylation in Arabidopsis thaliana seedlings after cryopreservation using methylation-sensitive amplified polymorphism. Of 662 fragments, 598 were shared by all the samples and their descendants; and among 64 different fragments, a varied 48 (75%) were genetically related to their descendants. Compared to the control, 14 sites showed de novo methylation, and 22 sites were demethylated in the samples after cryopreservation. Among samples treated with cryoprotection and after cryopreservation were 624 shared fragments and 38 fragments of difference, with 7.45% and 6.63% difference in the two treatment samples, respectively, as compared with the control. In conclusion, the results showed that whether cryostorage in liquid nitrogen or other treatments had impact on the DNA methylation states of the material in this study, and most of the change could be genetic to their descendants.

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[1] . [J]. Chinese Bulletin of Botany, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chinese Bulletin of Botany, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chinese Bulletin of Botany, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chinese Bulletin of Botany, 2004, 21(03): 360 -366 .
[5] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chinese Bulletin of Botany, 2000, 17(专辑): 261 .
[6] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chinese Bulletin of Botany, 1995, 12(01): 56 .
[7] . [J]. Chinese Bulletin of Botany, 1994, 11(专辑): 8 -9 .
[8] Yunpu Zheng;Jiancheng Zhao * ;Bingchang Zhang;Lin Li;Yuanming Zhang . Advances on Ecological Studies of Algae and Mosses in Biological Soil Crust[J]. Chinese Bulletin of Botany, 2009, 44(03): 371 -378 .
[9] Zili Wu, Mengyao Yu, Lu Chen, Jing Wei, Xiaoqin Wang, Yong Hu, Yan Yan, Ping Wan. Transcriptome Analysis of Physcomitrella patens Response to Cadmium Stress by Bayesian Network[J]. Chinese Bulletin of Botany, 2015, 50(2): 171 -179 .
[10] XU Zhen-Zhu, ZHOU Guang-Sheng, XIAO Chun-Wang, WANG Yu-Hui. INTERACIVE EFFECTS OF DOUBLED ATMOSPHERIC CO2 CONCENTRATIONS AND SOIL DROUGHT ON WHOLE PLANT CARBON ALLOCATION IN TWO DOMINANT DESERT SHRUBS[J]. Chin J Plan Ecolo, 2005, 29(2): 281 -288 .