Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (02): 178-184.doi: 10.3969/j.issn.1674-3466.2009.02.005

• 研究报告 • Previous Articles     Next Articles

Identification of Genes Differentially Expressed in Seeds of Brassica napus Planted in Nanjing and Lhasa by Arabidopsis Microarray

Sanxiong Fu, Cunkou Qi*   

  1. Institute of Industrial Crops of Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2008-08-26 Revised:2008-10-20 Online:2009-03-01 Published:2009-03-01
  • Contact: Cunkou Qi


Two Arabidopsis microarrays were used to analyze the differential expression of genes during seed development of a high-oil content Brassica napus cultivar, H105, planted in Nanjing (altitude: 8.9 m) and Lhasa (altitude: 3 658 m). The oil contents of H105 were (46.04±1.42)% in Nanjing and (53.09±1.35)% in Lhasa. We isolated the seeds 30 days after flowering at periods
preceding and including the major accumulation of storage oil. Transcript levels of 421 genes were altered two-fold or higher for H105 planted in Lhasa than in Nanjing. Of these, 192 showed up-regulated expression and 229 down-regulated expression. These differentially expressed genes were involved in different processes and had different functions, including metabolism, transportation, binding, transcription, structure, development, and signal transduction. Some key genes with differential expression, such as chlorophyll a-b binding protein, sucrose synthase, pyruvate kinase, 6-phosphogluconate dehydrogenase, ATP-citrate lyase, citrate synthase, isocitrate dehydrogenase, FAD6 and FAD7, relate to photosynthesis, sugar metabolism and fatty acid synthesis. These results reveal the expression profile of correlated genes in B. napus planted in two areas, and combined with results from others, suggest that the improvement of photosynthesis and sugar metabolism perhaps can increase lipid biosynthesis.

No related articles found!
Full text



[1] Liu Ying-di. The Role of Ultrastructure in Algal Systematics[J]. Chinese Bulletin of Botany, 1990, 7(04): 18 -23 .
[2] Fan Guo-qiang and Jiang Jian-ping. Study on the Methods of Extraction of Protein from Paulownia Leaves[J]. Chinese Bulletin of Botany, 1997, 14(03): 61 -64 .
[3] Tong Zhe and Lian Han-ping. Cryptochrome[J]. Chinese Bulletin of Botany, 1985, 3(02): 6 -9 .
[4] Huang Ju-fu and Luo Ai-ling. The Advances of the Studies on Extraction of FeMoco from Nitrogenase Molybdenum-Iron Protein[J]. Chinese Bulletin of Botany, 1991, 8(03): 19 -25 .
[5] Hsu Rong-jiang Gu Wen-mao Gao Jing-cheng and Peng Chang-ming. Inhibitory Effect of High CO2 and Low O2 Tension on Ethylene Evolution in Apples[J]. Chinese Bulletin of Botany, 1984, 2(01): 29 -31 .
[6] Zou Shu-hua;Zhao Shu-wen and Xu Bao. Electropheresis Profiles of Esterase Isozymes in Different Types of Soybean[J]. Chinese Bulletin of Botany, 1985, 3(06): 18 -20 .
[7] . [J]. Chinese Bulletin of Botany, 1999, 16(增刊): 49 -52 .
[8] Houqing Zeng, Yaxian Zhang, Shang Wang, Xiajun Zhang, Huizhong Wang, Liqun Du. Calcium/calmodulin-mediated Signal Transduction System in Plants[J]. Chinese Bulletin of Botany, 2016, 51(5): 705 -723 .
[9] Gu An-gen;Wang Mao and Wang Li-jun. Different Opinions on the Origins and Evolutions of Pteridophyte and Oymnosperms[J]. Chinese Bulletin of Botany, 1990, 7(02): 58 -62 .
[10] Xiaojuan Su, Baoguo Fan, Lichai Yuan, Xiuna Cui, Shanfa Lu. Selection and Validation of Reference Genes for Quantitative RT-PCR Analysis of Gene Expression in Populus trichocarpa[J]. Chinese Bulletin of Botany, 2013, 48(5): 507 -518 .