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Callus Induction and Plant Regeneration of Rosa hybrida

Huan Feng, Shuli Yi, Jiaheng Xie, Mengqi Lei, Xuan Huang*   

  1. Provincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, China
  • Received:2014-04-01 Revised:2014-06-20 Online:2014-07-02 Published:2014-09-01
  • Contact: Xuan Huang

Abstract: We used leaves (12-d-old) of Rosa hybrida as explants and MS medium to examine the effect of combinations and concentrations of growth regulators on callus, adventitious buds and root induction. The optimal medium for inducing calli was MS medium with 3.0 mg·L–12,4-D+1.0 mg·L–16-BA; 100% callus induction ratio was obtained. 6-BA played a key role in adventitious bud induction. Adventitious buds were regenerated from calli on MS medium containing 1.0 mg·L–16-BA, (0.05–0.5) mg·L–1NAA and (0.02–0.2) mg·L–1TDZ. The callus redifferentiation ratio was about 92.6% in MS medium supplied with 1.0 mg·L–16-BA+0.1 mg·L–1NAA+0.02 mg·L–1TDZ. Auxin is an important factor for inducing rooting; 100% rooting was obtained on 1/4 MS medium containing 0.1 mg·L–1NAA. We established a tissue culture and high-frequency regeneration system of R. hybrida.

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[1] . [J]. Chin Bull Bot, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chin Bull Bot, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chin Bull Bot, 2004, 21(03): 360 -366 .
[5] Shi Jian ming;Gui Yao-lin and Zhu Zhi-qing. Observation on Amitosis of Sugarbeet (Beta vulgaris) Petiole during Dedifferentiation in Vitro[J]. Chin Bull Bot, 1989, 6(03): 155 .
[6] . [J]. Chin Bull Bot, 1994, 11(专辑): 76 .
[7] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chin Bull Bot, 2000, 17(专辑): 261 .
[8] XU Jing-Xian WANG Yu-Fei YANG Jian PU Guang-Rong ZHANG Cui-Fen. Advances in the Research of Tertiary Flora and Climate in Yunnan[J]. Chin Bull Bot, 2000, 17(专辑): 84 -94 .
[9] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chin Bull Bot, 1995, 12(01): 56 .
[10] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 -9 .