Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (5): 587-594.doi: 10.3724/SP.J.1259.2014.00587

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The Establishment of Agrobacterium-mediated Transient Expression System in Cotton Cotyledons

Xuemei Liu, Lei Wang, Tianlong Wen, Peng He, Jianing Yu*   

  1. College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China
  • Received:2013-08-01 Revised:2013-11-16 Online:2014-07-02 Published:2014-09-01
  • Contact: Jianing Yu

Abstract: The plant transient expression system plays an important role in analysis of subcellular localization, interaction and promoter activity of protein. Here, we established a transient expression system in cotton cotyledons based on Agrobacterium-mediated transformation with GUS and eGFP as reporter genes. We investigated the optimal conditions for the system, including cotyledon growth time, Agrobacterium concentration, injection volume and co-culture time. The highest efficiency of exogenous gene expression was 50 μL Agrobacterium LBA4404 or GV3101 with OD600=0.5 and co-culture for 2–3 days in cotton cotyledons germinated for 6 days. The transient expression system is convenient and only needs 8 to 13 days to detect exogenous protein expression from germination to results. The system may have a good application for analysis of gene function in cotton.

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