Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (2): 139-149.doi: 10.3724/SP.J.1259.2014.00139

Previous Articles     Next Articles

Cloning and Expression Analysis of Calcium-dependent Phosphokinase Genes in Chenopodium album Under Stresses

Shasha Chen, Zhuanzhuan He, Shengxiu Jiang, Jiajia Xing, Xiuyun Lü, Haiyan Lan*   

  1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
  • Received:2013-02-04 Revised:2013-08-21 Online:2014-01-07 Published:2014-03-01
  • Contact: Haiyan Lan E-mail:lanhaiyan6908@gmail.com

Abstract: By homology cloning, we obtained the core sequences of 7 calcium-dependent protein kinase (CDPK) genes, CaCPK1 to CaCPK7, from salt-tolerant Chenopodium album. Quantitative real-time PCR revealed that CaCPK1, CaCPK2, CaCPK3 and CaCPK4 were significantly induced by salt stress with different patterns. The expression pattern of CaCPK1, CaCPK2 and CaCPK3 was also changed in C. album under both H2O2 and abscisic acid (ABA) stresses. Diphenylene iodonium (DPI, inhibitor of H2O2 synthesis) and sodium tungstate (Na2WO4, inhibitor of ABA synthesis) significantly inhibited the expression of CaCPK1, CaCPK2 and CaCPK3 on exposure to 300 mmol·L–1NaCl. We obtained the full-length sequences of 3 CDPKsCaCPK1, CaCPK2 and CaCPK3―by 3′- and 5′-RACE, which contain open reading frames of 1 632, 1 704, and 1 590 bp, respectively. The production of ABA and H2O2 induced by salt may be involved in induction of CaCPK1, CaCPK2 and CaCPK3, facilitating the analysis of the function of CDPK gene family in C. album.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Lu Zhong-shu. Plant Growth Regutators in Relation to Plant Water Status[J]. Chin Bull Bot, 1985, 3(04): 1 -6 .
[2] Li Da Jue;Han Yun-zhou and Wan Li-ping. Studies on Germplasm Collections of Carthamus tinctorius IV Screening of the characterization of Seed Domancy[J]. Chin Bull Bot, 1990, 7(02): 50 -52 .
[3] . [J]. Chin Bull Bot, 1999, 16(增刊): 45 -46 .
[4] Yang Hong-yuan. Basic Principle and Method of Fluorescence Microscopy[J]. Chin Bull Bot, 1984, 2(06): 45 -48 .
[5] LU Jin-Yao;LUO Ai-Ling and LIANG Zheng. Some Improvement of TD-PAGE Technology[J]. Chin Bull Bot, 1998, 15(03): 69 -72 .
[6] LI Ling-Hao and CHEN Zuo-Zhong. The Global Carbon Cycle in Grassland Ecosystems and Its Responses to Global Change I . Carbon Flow Compartment Model, Inputs and Storage[J]. Chin Bull Bot, 1998, 15(02): 14 -22 .
[7] Huanhuan Xu, Jian Kang, Mingxiang Liang. Research Advances in the Metabolism of Fructan in Plant Stress Resistance[J]. Chin Bull Bot, 2014, 49(2): 209 -220 .
[8] . [J]. Chin Bull Bot, 2013, 48(1): 4 -5 .
[9] . [J]. Chin Bull Bot, 1996, 13(专辑): 45 .
[10] SHU Qun-Fang;ZHOU Lu;LI Wen-Bin;ZHANG LI-Ming and SUN Yong-Ru. Study on Gel Electrophoresis of Protein from Plant and Our Improved Methods[J]. Chin Bull Bot, 1998, 15(06): 73 -78 .