Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (1): 78-86.doi: 10.3724/SP.J.1259.2014.00078

Previous Articles     Next Articles

Cytochemical Localization of ATPase and Acid Phosphatase in Minor Veins of the Leaf of Vicia faba During Different Developmental Stages

Li Wang1, Qinqin Wang2, Youqun Wang2*   

  1. 1College of Biological Science, China Agricultural University, Beijing 100193, China

    2State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100193, China
  • Received:2012-12-24 Revised:2013-04-11 Online:2014-01-01 Published:2014-01-01
  • Contact: Youqun Wang

Abstract: Using a lead precipitation technique, we investigated the cytochemical localization of adenosine triphosphatase (ATPase) and acid phosphatase (APase) in the minor veins of young leaves and in the sink and source regions of leaves undergoing sink-source transition in Vicia faba. The leaves undergoing sink-source transition were determined by the 6(5) carboxyfluorescein tracing. The plasmalemma and cell wall of transfer cells and the differentiating xylem elements in the young leaf showed intensive reaction products of ATPase and APase. The reaction products of ATPase and APase were weak in the plasmalemma of both transfer cells and sieve elements of the minor veins in the sink region. We observed a large number of ATPase and APase reaction products in the plasmalemma of both sieve elements and transfer cells. Thus, dynamic changes in the distribution of ATPase and APase occur at different developmental stages of minor veins. The role of ATPase and APase in apoplastic loading and differentiation of sieve and xylem elements is discussed.

No related articles found!
Full text



[1] Han Xian-zhong;Zhang Zhi-guo;Liu Hua and Zhao Li hong. Preliminary Research on the Growth Conditions of Adventitious Roots of GENTIANA MANSHUR CA[J]. Chin Bull Bot, 1990, 7(03): 49 -51 .
[2] Suxia Xu;Liangsheng Wang;Qingyan Shu;Minghua Su;Qingyun Huang;Wenhui Zhang;Gongshe Liu . Progress of Study of the Biology of the Resource Plant Bougainvillea[J]. Chin Bull Bot, 2008, 25(04): 483 -490 .
[3] . Advances on Plant Science Research in China in 2005[J]. Chin Bull Bot, 2006, 23(3): 225 -241 .
[4] Zhang Fu-ren and Mo Ri-gen. A Simple Technique for Observing Fracture Surface of Pollen Grains by SEM[J]. Chin Bull Bot, 1992, 9(03): 63 -64 .
[5] MA Yue-Ping CHEN Fan DAI Si-Lan. Studies of LEAFY Homologue Genes in Higher Plants[J]. Chin Bull Bot, 2005, 22(05): 605 -613 .
[6] . [J]. Chin Bull Bot, 1998, 15(专辑): 44 -45 .
[7] Jiang Gao-ming. A Study on the Historical Development Present Characteristics and Managements of the Urban Vegetation of Chengde City[J]. Chin Bull Bot, 1994, 11(04): 33 -38 .
[8] Chen Bao-li;Jin Xiao-ping and Zhang Qi-li. Specimen Preparation of Scanning Eletron Microscopy for Investigation on Development of Tapetum and Microspore[J]. Chin Bull Bot, 1989, 6(02): 124 -126 .
[9] Hongliang Tang Xueli He. Seed Coat Patterns in the Genus Hedysarum L.(Leguminosae) from China with Their Systematic Significance[J]. Chin Bull Bot, 2007, 24(05): 614 -619 .
[10] Liu Ming-jian Wang Jing-min Du Nai-qun and Kong Zhao-chen. A Preliminary Study on the Environmentae Changes in Huayin County, Shanxi Province Since Pleistocene Based on Pollen Analysis[J]. Chin Bull Bot, 1984, 2(01): 51 -54 .