Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (1): 78-86.doi: 10.3724/SP.J.1259.2014.00078

Previous Articles     Next Articles

Cytochemical Localization of ATPase and Acid Phosphatase in Minor Veins of the Leaf of Vicia faba During Different Developmental Stages

Li Wang1, Qinqin Wang2, Youqun Wang2*   

  1. 1College of Biological Science, China Agricultural University, Beijing 100193, China

    2State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100193, China
  • Received:2012-12-24 Revised:2013-04-11 Online:2014-01-01 Published:2014-01-01
  • Contact: Youqun Wang E-mail:wangyq@cau.edu.cn

Abstract: Using a lead precipitation technique, we investigated the cytochemical localization of adenosine triphosphatase (ATPase) and acid phosphatase (APase) in the minor veins of young leaves and in the sink and source regions of leaves undergoing sink-source transition in Vicia faba. The leaves undergoing sink-source transition were determined by the 6(5) carboxyfluorescein tracing. The plasmalemma and cell wall of transfer cells and the differentiating xylem elements in the young leaf showed intensive reaction products of ATPase and APase. The reaction products of ATPase and APase were weak in the plasmalemma of both transfer cells and sieve elements of the minor veins in the sink region. We observed a large number of ATPase and APase reaction products in the plasmalemma of both sieve elements and transfer cells. Thus, dynamic changes in the distribution of ATPase and APase occur at different developmental stages of minor veins. The role of ATPase and APase in apoplastic loading and differentiation of sieve and xylem elements is discussed.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] LIU Jun;ZHAO Lan-Yong;FENG Zhen;ZHANG Mei-Rong;WU Yin-Feng. Optimization Selection of Genetic Transformation Regeneration System from Leaves of Dendranthema morifolium[J]. Chin Bull Bot, 2004, 21(05): 556 -558 .
[2] Luo Jian-ping and Ja Jing-fen. Structure and Function of Plant Oligosaceaharins[J]. Chin Bull Bot, 1996, 13(04): 28 -33 .
[3] YANG Qi-He SONG Song-Quan YE Wan-HuiYIN Shou-HuaT. Mechanism of Seed Photosensitivity and FactorsInfluencing Seed Photosensitivity[J]. Chin Bull Bot, 2003, 20(02): 238 -247 .
[4] CUI Yue-Hua;WANG Mao and SUN Ke-Lian. Morphological Study of Gutta-containing Cells in Eucommia ulmoides Oliv.[J]. Chin Bull Bot, 1999, 16(04): 439 -443 .
[5] CHEN Shao-Liang LI Jin-Ke BI Wang-Fu WANG Sha-Sheng. Genotypic Variation in Accumulation of Salt Ions, Betaine and Sugars in Poplar Under Conditions of Salt Stress[J]. Chin Bull Bot, 2001, 18(05): 587 -596 .
[6] . Advances in Research into Low-Phytic-Acid Mutants in Crops[J]. Chin Bull Bot, 2005, 22(04): 463 -470 .
[7] Cong Ma, Weiwen Kong. Research Progress in Plant Metacaspase[J]. Chin Bull Bot, 2012, 47(5): 543 -549 .
[8] Chang’en Tian, Yuping Zhou. Research Progress in Plant IQ Motif-containing Calmodulin-binding Proteins[J]. Chin Bull Bot, 2013, 48(4): 447 -460 .
[9] Huawei Xu, Dianyun Hou. Research Advances in Protein Transport into Chloroplasts in Plant Cell#br#[J]. Chin Bull Bot, 2018, 53(2): 264 -275 .
[10] Li Jiandong, Zheng Huiying. ?ber die Anwendung der Braun-Blanquet's Methode in der Steppen-Untersuchung[J]. Chin J Plan Ecolo, 1983, 7(3): 186 -203 .