cDNA Cloning and Expression Patterns of SYP Genes in Manihot esculenta

doi:10.3724/SP.J.1259.2014.00063

Abstract

Abstract: Syntaxin protein (SYP) is a kind of vesicle-mediated transporter-related protein in plant cells. Several SYP genes were reported to respond to biotic and abiotic stress. To study the genetics, evolution and expression patterns of SYP genes in the tropical crop cassava (Manihot esculenta), we used bioinformatics to analyze gene structure, protein structure, nucleotide polymorphism and evolution and detected gene expression patterns by RT-PCR in different cassava tissues. The MeSYP gene structure and protein structure were conserved; cDNA sequences of SYP genes were highly consistent in different varieties and genes; rich nucleotide variations were regular between family members but were mainly synonymous mutations. Phylogenetic analysis revealed that plant SYP genes could be divided into 2 subfamilies. Cassava SYP genes tended to group with those of castor oil plant (Ricinus communis). SYP genes were together in the evolutionary tree, in the same branch. The 5 MeSYP members showed tissue-specific expression. Molecular characteristics of SYP protein in cassava, genetic evolutionary relationships and expression pattern analysis are valuable for revealing MeSYP functions and developing functional single nucleotide markers.

CLC Number:

Wenqi Li, Mengbin Ruan, Bin Wang, Pingjuan Zhao, Ming Peng. cDNA Cloning and Expression Patterns of SYP Genes in Manihot esculenta[J]. Chinese Bulletin of Botany, DOI: 10.3724/SP.J.1259.2014.00063.

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