Chin Bull Bot ›› 2014, Vol. 49 ›› Issue (1): 8-18.doi: 10.3724/SP.J.1259.2014.00008

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Solexa Sequencing and Bioinformatics Analysis of Small RNA in Winter Wheat

Ru Huang1, Jing Cang1*, Jing Yu1, Baowei Lu2, Lijie Liu1,3, Jianfei Wang1, Renming Guo1, Chen Xu1   

  1. 1College of Life Sciences, Northeast Agricultural University, Harbin 150030, China;

    2Heilongjiang Vocational Institute of Ecological Engineering, Harbin 150025, China;

    3College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China
  • Received:2013-03-29 Revised:2013-07-03 Online:2014-01-01 Published:2014-01-01
  • Contact: Jing Cang

Abstract: Dongnongdongmai 1 is the first cultivated winter wheat variety that can survive in Heilongjiang province. The cold tolerance of the tillering nodes directly affects the ability of overwintering. MicroRNAs (miRNAs) are a class of small non-coding endogenous RNAs with a critical role in development and environmental responses in plants. We analyzed the small RNAs of tillering nodes of Dongnongdongmai 1 under 5°C and –10°C. Under 5°C condition, we detected 2 229 955 unique sequences and 35 known miRNAs, which can be grouped into 30 miRNA families, with abundance ranging from 1 to 173 810. Under –10°C condition, we detected 3 721 449 unique sequences and 29 known miRNAs, which can be grouped into 24 miRNA families, with abundance ranging from 1 to 105 868. Target gene prediction revealed 53 putative target genes from the 30 miRNAs at 5°C, some of which were involved in transcription regulation, metabolism, stress response and signal transduction. Both types and abundance of known miRNAs differed in Dongnongdongmai 1 grown at 5°C and –10°C.

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