Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (6): 651-657.doi: 10.3724/SP.J.1259.2013.00651

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Spore Germination and Callus Induction of Atrichum undulatum

Xiaofeng Wu, Ruoyang Hu*, Xuedong Li   

  1. School of Life Sciences, Capital Normal University, Beijing 100048, China
  • Received:2012-09-12 Revised:2012-12-13 Online:2013-12-03 Published:2013-11-01
  • Contact: Ruoyang Hu E-mail:huruoyang@aliyun.com

Abstract: We studied the spore germination and protonema development of Atrichum undulatum collected from Wuling Mountain, Hebei Province (1 500 m a.s.l.). The dormancy stage is absent during spore development. Spores in incubation culture take 3 days to germinate. Spores germinate exogenously by forming a protonema in 2 growth phases: chloronema and caulonema. The best growth conditions were MS medium with 2% glucose at 25°C/20°C, 14 h light/10 h dark, 36 μmol·m–2·s–1. The optimal medium for callus induction is MS medium supplemented with 2% glucose and 1.0 mg?L–1 6-BA to obtain high-quality cormus for large-scale cultivation.

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