Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (5): 540-549.doi: 10.3724/SP.J.1259.2013.00540

Previous Articles     Next Articles

Histology and Development Analysis of Meristematic Nodules from Cultured Pulsatilla koreana

Yuji Lian*, Guangzhe Lin, Xiaomei Zhao   

  1. College of Life Sciences, Linyi University, Linyi 276005, China
  • Received:2012-08-27 Revised:2012-12-27 Online:2013-09-26 Published:2013-09-01
  • Contact: Yuji Lian E-mail:lianyuji@lyu.edu.cn

Abstract: Paraffin sections of Pulsatilla koreana petiole explants were studied to illustrate the microstructure of the meristematic nodule and plant regeneration pathway. Meristematic nodules could be induced from petiole explants on Murashige and Skoog medium containing 3 mg⋅L–1 zeatin with 0.5 mg⋅L–1 indole acetic acid. After 42 days of culture the frequency of nodule induction was 82.5%, and 95% of nodules converted to adventitious shoots after 56 days culture. Globular structures were observed after 14 days’ induction. Prenodular structures of cambial origin appeared from the calluses and gave rise to nodules; shoot buds formed after 28 days’ of culture. Nodules kept giving rise to sub-nodules, from which arose an increasing number of shoot buds. Most apical meristem structures with leaf primordia were observed after 42 days’ culture. Meristemoid regions and cells were much smaller in callus-like tissue than in surrounding cells. Some parts of the sections showed epidermal or sub-epidermal cells, vascular cambium, and meristemoid cells, which were regularly or irregularly distributed on the callus-like tissue. These structures provide evidence that callus-like tissue plays an important role in Pulsatilla species for shoot or root differentiation.

[1]黄泰康,尤啓冬,史美谣 (1994). 常用中药成分与药理手册. 北京:中国药学科技出版社:743
[2]廉玉姬,赵小梅,林光哲.朝鲜白头翁的组织培养与快繁技术[J].园艺学报,2010,37:491-498
[3]赵 渤,鲁新海 (2002). 药用花卉栽培和利用. 北京:中国农业出版社:311
[4]钟原, 成仿云, 秦磊.分生结节: 一种有价值的植株再生途径[J].植物学报,2011,46:350-360
[5]Aitken-Christie J, Singh AP (1988). Multiplication of meristematic t: a new t culture system for radiata pine. In: Hanover JW, Keathley DE (eds) Genetic manipulation of woody plants. Plenum, New York. pp. 413–432
[6]Bae KH (1999). The medicinal plants of Korea. Kyo-Hak Press 139
[7]Bang SC, Kim Y, Lee JH (2005a). Triterpenoid saponins from the roots of Pulsatilla koreana. J Nat Prod 68, 268–272
[8]Bang SC.[J].Lee JH, Song GY, Kim DH, Yoon MY, Ahn BZ (2005b). Antitumor activity of Pulsatilla koreana saponins and their structure activity relationship. Chem Pharm Bull,1451,53:-
[9]Batista D, Ascens?o L, Sousa MJ, Pais MS.Adventitious shoot mass production of hop (Humulus lupulus L[J].) var. eroica in liquid medium from organogenic nodule cultures. Plant Sci,2000,151:47-57
[10]Cho SC, Zakir SM, Moon SS.Anti-acne activities of pulsaquinone, hydropulsaquinone, and structurally related 1, 4-quinone derivatives[J].Arch Pharm Res,2009,32:489-494
[11]Ferreira S, Batista D, Serrazina S, Pais MS.Morphogenesis induction and organogenic nodule differentiation in Populus euphratica Oliv[J].leaf explants. Plant Cell T Organ Cult,2009,96:35-43
[12]George EF, Hall MA, de Klerk GJ (2008). Plant propagation by t culture, 3rd edn, Vol 1, The Background. Dordrecht: Springer. pp. 1–64
[13]Godo T, Kobayashi K, Tagami T, Matsui K, Kida T.In vitro propagation utilizing suspension cultures of meristematic nodular cell clumps and chromosome stability of Lilium ?ormolongi hort[J].Sci Hortic,1998,72:193-202
[14]Jung SJ, Jeong JH, Yoon ES, Choi YE (2007). Plant regeneration from callus and adventitious root segments of Pulsatilla koreana Nakai. J Plant Biotechnol 34:153–159
[15]Ko JA, Kim HS.Effective micropropagation of Pulsatilla cernua var koreana through apical meristem culture[J].Korean J Plant Res,2008,21:362-367
[16]Ko JA, Kim HS.Microspore-derived embryogenesis and plant regeneration from anther culture of Pulsatilla cernua var[J].koreana. Korean J Hort Sci Technol,2006,24:290-295
[17]Kongbangkerd A, K?pf A, Allacher P, Wawrosch C, Kopp B.Micropropagation of squill (Charybdis numidica) through nodule culture[J].Plant Cell Rep,2005,23:673-677
[18]Lee HS, Beon MS, Kim MK.Selective growth inhibitor toward human intestinal bacteria derived from Pulsatilla cernua root[J].J Agric Food Chem,2001,49:4656-4661
[19]Lee MS, Oh KH.Histological studies on in vitro propagation of Pulsatilla koreana Nakai[J].Korean J Med Crop Sci,1993,1:137-157
[20]Lin GZ, Zhao XM, Hong SK, Lian YJ.Somatic embryogenesis and shoot organogenesis in the medicinal plant Pulsatilla koreana Nakai[J].Plant Cell T Organ Cult,2011,106:93-103
[21]McCown BH, Zeldin EL, Pinkalla HA, Dedolph RR (1988). Nodule culture: a developmental pathway with high potential for regeneration, automated micropropagation and plant metabolite production from woody plants. In: Hanover JW, Keathley DE, eds. Genetic manipulation of woody plants. New York & London: Plenum Press. pp. 149–166
[22]Mimaki Y, Kuroda M, Asano T.Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells[J].J Nat Prod,1999,62:1279-1283
[23]Murashige T, Skoog F.A revised medium for rapid growth and bioassays with tobacco t culture[J].Physiol Plant,1962,15:473-497
[24]Piéron S, Dekegel D, Boxus P.Histological study of nodule morphogenesis from Cichorium intybus L[J].leaves cultivated in vitro. In Vitro Cell Dev Biol —Plant,1998,34:87-93
[25]Quoirin M, Vieira RC.Rhizogenesis and nodule formation from callus of Eucalyptus grandis and E[J].grandis xurophylla. Arq. Biol. Tecnol,1995,38:793-798
[26]Salaj J, Petrovská B, Obert B, Pret’ová A.Histological study of embryo-like structures initiated from hypocotyl segments of flax (Linum usitatissimum L[J].). Plant Cell Rep,2005,24:590-595
[27]Sang CK, Kim EH, Kim HY.Germination and life span of Pulsatilla cenua var[J].koreana seeds. J Kor Soc Hort Sci,1993,34:207-212
[28]Te-chato S, Lim M.Improvement of mangosteen micropropagation through meristematic nodular callus formation from in vitro-derived leaf explants[J].Sci Hortic,2000,86:291-298
[29]Teng WL.An alternative propagation method of Ananas through nodule culture[J].Plant Cell Rep,1997,16:454-457
[30]Warrag E, Lesney MS, Rockwood DJ.Nodule culture and regeneration of Eucalyptus grandis hybrids[J].Plant Cell Rep,1991,9:586-589
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Qin Wei-cheng Li Jian-zhong. The Application Effects of the Cold-resister CR-4 in Our Area's Rice Seedling Culture[J]. Chin Bull Bot, 1994, 11(特辑): 102 -104 .
[2] Ningguang Dong, Ying Gao, Wei Wang, Weilun Yin, Dong Pei. Immunogold Silver Localization of Indole-3-acetic Acid (IAA) During the Rhizogenesis of In Vitro Poplar[J]. Chin Bull Bot, 2011, 46(3): 324 -330 .
[3] HONG Wei CAO Jia-Shu. The Function of FLC in Vernalization Process[J]. Chin Bull Bot, 2002, 19(04): 406 -411 .
[4] . Development and Utilization of Plant Resources II[J]. Chin Bull Bot, 1994, 11(02): 53 -57 .
[5] FAN Qing-Shu ZHAO Jian-Cheng YU Shu-Hong LI Xiu-Qin. Progress in Study on Spore Germination and Protonema Development of the Bryophytes[J]. Chin Bull Bot, 2003, 20(03): 280 -286 .
[6] LIU Jian-Wu LIU Ning. The Progress in Study on Development of Fern Gametophytes and Differentiation of Sex Organ[J]. Chin Bull Bot, 2001, 18(02): 149 -157 .
[7] An Cheng-xi. Studies on the Chemical Constituents of Essential of Aiania-Tanuifolia[J]. Chin Bull Bot, 1997, 14(增刊): 74 -76 .
[8] Nie Wei. Observation on some Biological Characteristics of Juncellus serotinus in Transplanted Rice[J]. Chin Bull Bot, 1988, 5(01): 34 -36 .
[9] . Mechanism of Plant Photosynthetic Acclimation to Elevated Atmospheric CO2[J]. Chin Bull Bot, 2005, 22(04): 486 -493 .
[10] WEI Ze-Xiu, LIANG Yin-Li, YAMADA Satoshi, ZENG Xing-Quan, ZHOU Mao-Juan, HUANG Mao-Lin, WU Yan. RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS[J]. Chin J Plan Ecolo, 2009, 33(3): 580 -586 .