Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (2): 145-150.doi: 10.3724/SP.J.1259.2013.00145

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Expression Analysis of MwAG in Different Organs and Developmental Stages of Magnolia wufengensis

Danlong Jing1, Jiang Ma1, Bo Zhang1, Yiyang Han1, Zhixiong Liu2, Faju Chen1*   

  1. 1Biotechnology Research Center, China Three Gorges University, Yichang 443002, China;

    2College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
  • Received:2012-09-12 Revised:2012-12-26 Online:2013-04-07 Published:2013-03-01
  • Contact: Faju Chen E-mail:chenfj616@163.com

Abstract: The function gene MwAG as a key transcription factor that plays crucial roles in the development of Magnolia wufengensis by regulating the development of the stamen and carpel. Semi-quantitative RT-PCR, Northern blot and quantitative RT-PCR methods were used to identify the spatial and temporal expression patterns of MwAG in M. wufengensis at several important stages of the morphological differentiation of flower buds. MwAG was expressed specially in the stamen and pistil, with no detectable expression in outer and inner whorls of the perianth and leaves. The expression was highest at the primordium and mature stages of stamen and pistil during morphological differentiation of organs. The expression peaked earlier in the stamen than in the pistil, and the time of expression was the same as with morphological differentiation of the stamen and pistil. At the early stage of the differentiation of flower buds, the expression was low when flower buds had more petal numbers of the same size, and the time of expression was consistent with the development and differentiation of the stamen and pistil in different flower buds (i.e., the more the petal numbers, the later the time of differentiation of stamen and pistil). MwAG may play a crucial role in stamen and carpel development of M. wufengensis.

贺随超,马履一,陈发菊 (2007). 红花玉兰种质资源遗传多样性初探[J]. 西北植物学报, 27(12): 2421-2428.
贺随超,马履一 (2008). 红花玉兰与玉兰亚属几个种亲缘关系的AFLP分析[J]. 植物研究, 28(3): 288-292.
王晓玲,马履一,贾忠奎,朱仲龙,张田田 (2011). 红花玉兰研究进展[J]. 北方园艺, (16): 202-205.
王罗荣, 马履一, 王希群, 贺随超, 刘鑫 (2007). 红花玉兰播种育苗技术的初步研究[J]. 浙江林学院学报, 24(2): 242-246.
贺窑青,马履一, 桑子阳 (2010). 红花玉兰花色形成的初步研究[J]. 西北植物学报, 30(11): 2252-2257.
曾英, 胡金勇, 李志坚 (2011). 植物MADS盒基因与花器官的进化发育[J]. 植物生理学通讯, 37(4): 281-287.
Coen ES, Meyerowitz EM (1991). The war of the whorls: genetic inter actions controlling flower development[J]. Nature, 353: 31-37.
Richard G.H. Immink, Kerstin Kaufmann, Gerco C. Angenent (2010). The ‘ABC’ of MADS domain protein behaviour and interactions[J]. Seminars in Cell & Developmental Biology, 21: 87-93.
Wentao Wu, Faju Chen, Danlong Jing, Zhixiong Liu, Lvyi Ma (2012). Isolation and Characterization of an AGAMOUS-Like Gene from Magnolia wufengensis(Magn oliaceae)[J]. Plant Mol Biol Rep, 30: 690-698.
Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2—△△Ct method. Methods, 25(4): 402-408.
Mark P. Running, Elliot M. Meyerowitz (1996). Mutations in the PERIANTHIA gene of Arabidopsis specifically alter floral organ number and initiation pattern[J]. Development, 122: 1261-1269.
Ying Wang, Xiaomei Zhang, Zhixiong Liu, Dandan Zhang, Jinzi Wang, Di Liu , Fenglan Li, Hai Lu (2012). Isolation and characterization of an AGAMOUS -like gene from Hosta plantaginea[J]. Mol Biol Rep , 39, (3): 2875-2881.
Annick Dubois, Olivier Raymond, Marion Maene, Sylvie Baudino, Nicolas B. Langlade, Ve′roniqueBoltz, Philippe Vergne, Mohammed Bendahmane (2010). Tinkering with the C-Function: A Molecular Frame for the Selection of Double Flowers in Cultivated Roses[J]. PLoS ONE, 5(2): e9288.
Kelsey D. Galimba, Theadora R. Tolkin, Alessandra M. Sullivan, Rainer Melzer, Günter Thei?en,and Verónica S. Di Stilio (2012). Loss of deeply conserved C-class floral homeotic gene function and C- and E-class protein interaction in a double-flowered ranunculid mutant. Proc Natl Acad Sci U S A. 109,(34):E2267-75.
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