Chinese Bulletin of Botany ›› 2013, Vol. 48 ›› Issue (1): 23-33.DOI: 10.3724/SP.J.1259.2013.00023

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Cloning and Characterization of an AP2/EREBP Gene TmAP2-1 from Tetraena mongolica

Yujing Wang1,2, Minchun Li1,2, Wang Wu1,2, Hanying Wu1, Yinong Xu1*   

  1. 1Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

    2Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-02-23 Revised:2012-04-13 Online:2013-01-01 Published:2012-11-01
  • Contact: Yinong Xu

Abstract: AP2/EREBP transcription factors play important roles in plant morphology, development, and stress responses. We cloned and characterized a putative AP2/ERF domain-containing gene from Tetraena mongolica by homology-based cloning and rapid amplification of cDNA ends. This gene, designated TmAP2-1 (accession no. JQ676996), encodes a protein of 483 amino acids with 2 copies of AP2 DNA binding domain. Subcellular localization analysis revealed that TmAP2-1 protein localized in the nucleus. TmAP2-1 protein has no transactivation activity in yeast. The expression of TmAP2-1 is induced by cold, salt, polyethylene glycol and abscisic acid. TmAP2-1 is detected in roots, shoots and leaves of T. mongolica, with the highest expression in leaves. Overexpression of TmAP2-1, accumulation of more soluble sugars in T. mongolica callus, but in reduced lipid content. TmAP2-1 probably represents an AP2/EREBP gene that plays a regulatory role in tolerance of T. mongolica to abiotic stresses by affecting accumulation of soluble sugars.

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